MITOCHONDRIAL/STRUCTURAL CORE

线粒体/结构核心

• 批准号: 6783181
• 负责人: Charles Leslie Hoppel
• 金额: $ 13.14万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6783181
• 关键词: apoptosis; biomedical facility; cell component structure /function; electron microscopy; histochemistry /cytochemistry; immunocytochemistry; light microscopy; mitochondria; molecular biology information system; myocardial ischemia /hypoxia; myocardium; reperfusion; tissue /cell preparation

The Mitochondrial/Structural Core (9004) provides support to all of the individual projects in the program. All projects have utilized and continue to require populations of SSM and IFM for biochemical study. All of the projects require the assessment of myocardial and/or mitochondrial morphology for experimental data as well as for the assessment of quality control. The Core provides the following services to all the projects: . Isolation of subsarcolemmal and interfibrillar mitochondria from experimental hearts. . Samples of in situ myocardium and isolated populations of SSM and IFM obtained for light and electron microscopy--the latter includes transmission, scanning, and high-voltage electron microscopy-- will provide quality control. . Excess tissue and mitoehondrial samples are catalogued and stored for future use. . Experiments are entered into a common database available via network to all projects. Samples and the results of studies of respiration and biochemical endpoints are collated via database. Myocardial pathology is studied by light microscopy. Histochemical, immunohistological, and electron immunoeytochemical studies are performed on myocardium before and following ischemia and reperfusion. The frequency of myocyte apoptosis following ischemia and reperfusion is assessed.

线粒体/结构核心(9004)为该计划中的所有单独项目提供支持。所有项目都利用并继续需要SSM和IFM种群进行生化研究。所有这些项目都需要评估实验数据的心肌和/或线粒体形态，以及评估质量控制。 核心为所有项目提供以下服务： 。实验性心脏肌膜下和纤维间线粒体的分离。 。光镜和电子显微镜获得的原位心肌样本和分离的SSM和IFM群体--后者包括透射式、扫描式和高压电子显微镜--将提供质量控制。 。多余的组织和线粒体样本被编入目录并储存起来，以备将来使用。 。实验被输入一个公共数据库，所有项目都可以通过网络获得。 呼吸作用和生化终点研究的样本和结果通过数据库进行整理。光镜下观察心肌病理变化。对缺血和再灌流前后的心肌进行组织化学、免疫组织学和电子免疫细胞化学研究。心肌细胞的凋亡率 对缺血和再灌流后进行评估。