CORE--ANALYTICAL

分析核心将为以下组件提供分析支持 该项目需要测定磷脂和它们的 血浆或组织中的衍生物。它还提供了分析支持 对那些从事蛋白质老化修饰研究的项目 和/或缺血再灌注，通过提供分子量和氨基酸 电喷雾-质谱法测定多肽的序列。 这一核心使PPG的研究人员能够研究脂肪酸， 磷脂和修饰蛋白质，没有时间和金钱成本， 进行这项工作所需的专业知识和分析设备 分析活动。 具体目标是： 1.提供气相色谱和气相色谱-质谱仪 非酯化脂肪酸馏分AS的脂肪酸组成分析 以及从复杂的脂肪组分(主要是磷脂) 水解液。 2.提供脂类的硅胶柱层析分级分离 磷脂的高效液相色谱分析 上课。此外，不寻常的结构确认/建立 用电喷雾质谱分析酰基肉碱和磷脂。 3.提供分子量和氨基酸序列测定 利用电喷雾质谱仪对蛋白质和/或多肽进行修饰。 4.为符合以下条件的程序提供额外的分析支持 CORE拥有资源和专业知识，即使通过程序也是如此 特定项目的范围有限。一个这样的例子是HIGH 2-巯基丙酰甘氨酸的高效液相色谱分析。

The Analytical Core will provide analytical support to those components of the project which required the measurement of phospholipids and their derivatives in plasma or tissue. It also provides the analytical support to those projects engaging in the study of protein modification from aging and/or ischemia reperfusion by providing molecular weight and amino acid sequence determination of peptides using electrospray mass spectrometry. This Core enables the PPG investigators to study fatty acids, phospholipids, and modified proteins without the time and monetary costs, expertise, and analytical equipment necessary for undertaking this analysis activity. The specific aims are: 1. Provide gas chromatographic and gas chromatograph-mass spectrometric analysis of fatty acid profiles from non esterified fatty acid fraction as well as from complex lipid fractions (mainly phospholipids) after hydrolysis. 2. Provide silica gel column chromatographic class separation of lipids and high performance liquid chromatographic analysis of phospholipid classes. In addition, the structural confirmation/establishment of unusual acylcarnitines and phospholipids using electrospray mass spectrometry. 3. Provide molecular weight and amino acid sequence determination of modified protein and/or peptides using electrospray mass spectrometry. 4. Provide additional analytical support for those procedures that the Core has the resources and expertise even through the procedures are of limited scope in a particular project. One such example is the high performance liquid chromatographic analysis of 2-mercaptopropionylglycine.