In vivo dynamics of presynaptic domains

突触前域的体内动力学

• 批准号: 6994085
• 负责人: ROBBY M WEIMER
• 金额: $ 4.99万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6994085
• 关键词: axon; cell morphology; chimeric proteins; electroporation; fluorescent dye /probe; genetically modified animals; green fluorescent proteins; ionophores; laboratory mouse; laboratory rat; neocortex; nerve endings; neural plasticity; neurogenesis; postdoctoral investigator; protein localization; synapses; synaptic vesicles; synaptophysin; tissue /cell culture; transfection /expression vector

DESCRIPTION (provided by applicant): Synaptic connectivity -- that is, synapse number and strength -- determines the computational power of neural networks. This begs the question; how are synaptic connections made, maintained and modified to accomplish the computational power of the mammalian brain? To address this question, I will image presynaptic proteins tagged with the green fluorescent protein (GFP), and its photoactivatable derivative (paGFP), in vivo and measure the following fundamental parameters as a function of development and experience: 1) How do axons branch and what fraction of new axonal branches are maintained into adulthood? 2) What fraction of presynaptic terminals formed during development are maintained into adulthood? 3) Does synaptic activity correlate with synaptic maintenance? These experiments will represent firsts in the characterization of cortical neurons in vivo during development and in the tracking of individual protein populations in vivo -- methodologies which will bridge the gap between molecular and cellular neurophysiology.

描述（由申请人提供）：突触连接性-即突触数量和强度-决定了神经网络的计算能力。这就引出了一个问题：突触连接是如何建立、维持和修改的，以实现哺乳动物大脑的计算能力？为了解决这个问题，我将图像标记的突触前蛋白质的绿色荧光蛋白（GFP），其光活化衍生物（paGFP），在体内和测量以下基本参数作为一个功能的发展和经验：1）如何做轴突分支和新的轴突分支的比例保持到成年？2)在发育过程中形成的突触前终末中有多少部分能维持到成年期？3)突触活动与突触维持相关吗？这些实验将代表在发育过程中的皮质神经元在体内的表征和在体内的单个蛋白质群体的跟踪中的第一次--这些方法将弥合分子和细胞神经生理学之间的差距。