Consumer and environmental microbiome effects of domestic probiotic cleaning products

据报道，芽孢杆菌属芽孢杆菌的芽胞。清洁产品中提供的细菌可以在家庭干燥的表面上发芽，产生繁殖型细菌，这种细菌可以通过竞争排斥处理过的表面来抵消病原体的生长(Caselli等人)。2016)。我们将测试在家庭环境中应用MBCP的程度：i)导致家庭中持续的芽孢杆菌定植。Ii)改变涂布表面的微生物区系。Iii)通过直接接触(厨房表面)或空中途径向消费者转移。IV)通过直接接触或通过定植表面修饰人(皮肤)微生物群。目的和目的：1)调查MBCP内微生物对国产硬表面材料的定植情况。这将包括评估孢子数量、活力、表面定植和在模拟家庭环境中重复使用MBCP到表面的持久性。这将使用高通量扩增序列测定、qPCR和细菌培养相结合的方法来完成。2)评估使用MBPC时国内表面微生物群的变化程度，包括任何观察到的变化的持续时间，以及它们的可逆性。微生物组分析将通过高通量扩增序列进行。将包括单叠氮丙啶治疗，以区分来自活细菌和非活细菌的DNA(Marotz等人，2018年；Ni等人，2019年；Sielaff等人，2019年)。3)调查MBCP内的孢子转移到厨房使用者的可能性。在受控的实验室环境中，使用厨房表面材料的样品来完成。4)利用(3)中产生的数据，设计和进行一项人体志愿者研究，研究直接(在部署期间)和间接(在食物准备期间)接触MBPC对皮肤微生物群的影响。5)利用(2)和(4)中产生的数据建立共生网络模型，研究MBCP对微生物群落结构的影响。将使用指数随机图模型框架对这些数据进行分析(Lusher等人，2013年)。这样的模型可以深入了解微生物群的稳定性和生态动力学(DeVries等人。2018年)。

It has been reported that spores of Bacillus spp. delivered in cleaning products can germinate on dry surfaces in the home, generating vegetative forms of bacteria, which can counteract the growth of pathogens by competitive exclusion on treated surfaces (Caselli et al. 2016). We will test to what extent MBCP application in domestic settings could: i) Lead to persistent Bacillus colonisation of the home. ii) Alter the microbiota of the application surfaces. iii) Transfer to consumers via direct contact (kitchen surfaces) or the airborne route. iv) Modify the human (skin) microbiome through direct contact or via colonised surfaces.Aim and Objectives: 1) To investigate the colonisation of domestic hard surface materials by microorganisms within MBCP. This will include an assessment of spore numbers, viability, surface colonisation and persistence in a simulated domestic environment during repeated application of MBCP to surfaces. This will be done using a combination of high throughput amplicon sequencing, qPCR and bacterial culture. 2) To evaluate the extent of the change in the domestic surface microbiome when using MBPC, including the duration of any observed changes, and their reversibility. Microbiome analyses will be done by high throughput amplicon sequencing. Propidium monoazide treatment will be included to differentiate between DNA derived from viable and non-viable bacteria (Marotz et al., 2018; Ni et al., 2019; Sielaff et al., 2019). 3) To investigate the potential for spores within MBCP to transfer to kitchen users. To be done using samples of kitchen surface materials within a controlled laboratory environment. 4) To use data generated in (3) to design and conduct a human volunteer study of the skin microbiome effects of both direct (during deployment) and indirect (during food preparation) contact with MBPC. 5) To use data generated in (2) and (4) to create co-occurrence network models, the impact of MBCPs on microbial community structure. These will be analysed using an exponential random graph model framework (Lusher et al 2013). Such models can be insightful into the stability and ecological dynamics of microbiomes (DeVries et al. 2018).