Correlative 3D Cryo-Imaging of Microorganisms for Understanding Anti- Microbial Agents

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This research will employ high spatio-temporal correlative 3D cryo-imaging to understand how individual AMA affects the cell {e.g. peroxidation, lipid plasticisation, chelation of multivalent cations bound to the lipids and permeation of small molecules through it) and to identify the best combination for fast kill. Cryo-FIB/SEM/TEM at Imperial and at the electron Bio Imaging Centre (eBIC) and cryo-Soft X-ray Tomography (SXT)/cryo-Structure Illumination Microscopy (SIM) at Diamond Light Source will be employed to understand AMA's mode of action and how the cells provide resistance, if any.