Vaculogenesis and renal mesodermal progenitor cells

血管生成和肾中胚层祖细胞

• 批准号: 7070006
• 负责人: MATTHEW D PLOTKIN
• 金额: $ 13.38万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7070006
• 关键词: cell differentiation; cell fusion; cell proliferation; developmental genetics; disease /disorder model; gene expression; genetically modified animals; injury; kidney cell; kidney disorder; laboratory mouse; mesoderm; microarray technology; pluripotent stem cells; regeneration; renal tubular transport; vascular endothelium

DESCRIPTION (provided by applicant): Dr. Matthew Plotkin is a Research Assistant Professor in the Dept. of Medicine at New York Medical College. Following clinical and research training in nephrology and several years in nephrology practice, Dr. Plotkin is currently engaged in basic science research focusing on the molecular biology of adult renal stem and progenitor cells with the long term goal of becoming an independent research scientist in an academic nephrology department. Dr. Plotkin is a member of a lab investigating various aspects of vascular biology relating to renal disease under the direction of Dr. Michael Goligorsky. As a member of the New York Medical College Nephrology Dept., responsibilities also include clinical work and interaction with both clinical and research trainees. Dr. Plotkin is interested in developing expertise in the biology of adult kidney stem cells and their role in microvascular and tubular repair following renal injury. The proposed research project involves study of the differentiation potential of multipotent mesodermal stem cell clones isolated from adult kidneys. In vitro vasculogenesis models will be used to determine the key molecules for inducing vessel formation and maturation from renal vascular progenitor cells. Studies will focus on the effects of cytokines and other molecules known to be activated in renal disease on progenitor cell proliferation and differentiation and identifying novel genes activated or deactivated during progenitor cell differentiation using cDNA microarray analysis. Animal models of vasculogenesis and tissue repair following renal injury will also be used to study the in vivo differentiation potential of these cells. Finally, both in vivo and in vitro studies will be done to determine if progenitor cells are capable of repairing damaged kidney cells by cell fusion as shown in other organs.

描述（由申请人提供）： 马修·普洛特金博士是纽约医学院医学系的研究助理教授。经过肾脏病学临床和研究培训以及几年的肾脏病学实践后，Plotkin 博士目前从事基础科学研究，重点关注成体肾干细胞和祖细胞的分子生物学，其长期目标是成为肾脏病学学术部门的独立研究科学家。 Plotkin 博士是在 Michael Goligorsky 博士的指导下研究与肾脏疾病相关的血管生物学各个方面的实验室的成员。作为纽约医学院肾病科的成员，职责还包括临床工作以及与临床和研究实习生的互动。 Plotkin 博士有兴趣发展成体肾干细胞生物学及其在肾损伤后微血管和肾小管修复中的作用方面的专业知识。拟议的研究项目涉及从成人肾脏中分离出的多能中胚层干细胞克隆的分化潜力的研究。体外血管发生模型将用于确定诱导肾血管祖细胞血管形成和成熟的关键分子。研究将集中于已知在肾脏疾病中激活的细胞因子和其他分子对祖细胞增殖和分化的影响，并使用 cDNA 微阵列分析识别在祖细胞分化过程中激活或失活的新基因。肾损伤后血管生成和组织修复的动物模型也将用于研究这些细胞的体内分化潜力。最后，将进行体内和体外研究，以确定祖细胞是否能够通过细胞融合修复受损的肾细胞，如其他器官所示。