Vaculogenesis and renal mesodermal progenitor cells

血管生成和肾中胚层祖细胞

• 批准号: 7410061
• 负责人: MATTHEW D PLOTKIN
• 金额: $ 13.53万
• 依托单位: NEW YORK MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-01 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7410061
• 关键词: Adult; Animal Model; Basic Science; Biology; Blood Vessels; Cell Differentiation process; Cell Proliferation; Cell fusion; Cells; Clinical; Clinical Research; Condition; Epithelial Cells; Genes; Genetic Transcription; Goals; Growth Factor; Implant; In Vitro; Injury; Intra-Arterial Infusions; Investigation; Kidney; Kidney Diseases; Label; Medical; Medicine; Microarray Analysis; Modeling; Molecular Biology; Mus; Natural regeneration; Nephrectomy; Nephrology; New York; Operative Surgical Procedures; Organ; Reperfusion Injury; Research; Research Project Grants; Research Training; Role; Scientist; Smooth Muscle; Smooth Muscle Myocytes; Stem Cell Development; Stem cells; System; Tissues; Tubular formation; Work; Wound Healing; cDNA Arrays; college; cytokine; implantation; in vivo; interest; kidney cell; kidney vascular structure; member; novel; professor; progenitor; repaired; stem; vasculogenesis

DESCRIPTION (provided by applicant): Dr. Matthew Plotkin is a Research Assistant Professor in the Dept. of Medicine at New York Medical College. Following clinical and research training in nephrology and several years in nephrology practice, Dr. Plotkin is currently engaged in basic science research focusing on the molecular biology of adult renal stem and progenitor cells with the long term goal of becoming an independent research scientist in an academic nephrology department. Dr. Plotkin is a member of a lab investigating various aspects of vascular biology relating to renal disease under the direction of Dr. Michael Goligorsky. As a member of the New York Medical College Nephrology Dept., responsibilities also include clinical work and interaction with both clinical and research trainees. Dr. Plotkin is interested in developing expertise in the biology of adult kidney stem cells and their role in microvascular and tubular repair following renal injury. The proposed research project involves study of the differentiation potential of multipotent mesodermal stem cell clones isolated from adult kidneys. In vitro vasculogenesis models will be used to determine the key molecules for inducing vessel formation and maturation from renal vascular progenitor cells. Studies will focus on the effects of cytokines and other molecules known to be activated in renal disease on progenitor cell proliferation and differentiation and identifying novel genes activated or deactivated during progenitor cell differentiation using cDNA microarray analysis. Animal models of vasculogenesis and tissue repair following renal injury will also be used to study the in vivo differentiation potential of these cells. Finally, both in vivo and in vitro studies will be done to determine if progenitor cells are capable of repairing damaged kidney cells by cell fusion as shown in other organs.

描述(由申请人提供)： 马修·普洛特金博士是该系的研究助理教授。纽约医学院医学院医学系。经过肾脏病临床和研究培训以及多年的肾脏病实践，普洛特金博士目前从事基础科学研究，专注于成人肾脏干细胞和前体细胞的分子生物学，长期目标是成为学术肾病科的独立研究科学家。普洛特金博士是一个实验室的成员，该实验室在迈克尔·戈里戈尔斯基博士的指导下，研究与肾脏疾病相关的血管生物学的各个方面。作为纽约医学院肾病学系的一员，其职责还包括临床工作以及与临床和研究实习生的互动。普洛特金博士有兴趣发展成人肾干细胞生物学及其在肾损伤后微血管和肾小管修复中的作用方面的专业知识。拟议的研究项目涉及从成人肾脏分离的多潜能中胚层干细胞克隆的分化潜力的研究。体外血管生成模型将被用来确定诱导肾血管前体细胞血管形成和成熟的关键分子。研究将集中在细胞因子和其他已知在肾脏疾病中被激活的分子对祖细胞增殖和分化的影响，以及利用基因芯片分析识别在祖细胞分化过程中激活或失活的新基因。肾损伤后血管生成和组织修复的动物模型也将用于研究这些细胞的体内分化潜力。最后，将进行体内和体外研究，以确定祖细胞是否能够像在其他器官中所显示的那样，通过细胞融合修复受损的肾脏细胞。