CORE--ANIMAL MODEL

动物模型将提供所有使用的小鼠，并负责订购小鼠，进行诱导实验性结肠炎所需的各种操作，维护屏障设施，进行常规调查以确保殖民地中不存在病原体，并协助 从这些小鼠身上获取组织。这些功能的集中提供了在不同项目中研究的小鼠的一致性和质量控制，降低了成本， 通过确保在项目中得到最佳利用，保存所需的小鼠数量。第二个目标是由特伦顿舍布博士进行集中病理分析， 兽医病理专家将对来自不同项目的组织切片进行编码， 必要时进行解释和定量评分。这有助于项目之间的互动以及结果的比较。第三个目的是提供用于实验的遗传修饰的转基因和敲除小鼠品系。这种基因改变的菌株是确定特定基因产物在复杂生物系统中的作用的强大分析工具，并且已经成为允许通过实验解决以前无法解决的基本问题的“试剂”。诱发突变的小鼠经常 为了进行最佳研究，必须在其他背景品系上繁殖，这需要通过鉴定一个或多个目标遗传元件来筛选每一代中的个体动物。查尔斯·埃尔森博士将担任主任，凯西韦弗博士将担任联合主任。小鼠将在屏障条件下圈养在与新Shelby研究大楼物理连接的新动物园中。来自比较医学系的Sam Gartner博士将担任顾问，由每个项目的PI组成的监督委员会将与Schoeb博士和Gartner一起监督使用和功能。

Animal Model will provide all mice used and it will be responsible for ordering mice, performing the various manipulations required to induce experimental colitis, maintaining a barrier facility, performing routine surveys to ensure absence of pathogens in the colony, and assist in harvesting tissues from such mice. Centralization of these functions provides uniformity and quality control of mice being studied in the various projects, reduces cost, and will help conserve numbers of mice required by ensuring their optimal use in the projects. A second aim is that of centralized pathologic analyses which will be performed by Dr. Trenton Schoeb, an expert veterinary pathologist. Tissue sections from the various projects will be coded prior to interpretation and quantitative scoring where needed. This facilitates interactions among the projects and also the comparison of results. A third purpose is to provide genetically modified transgenic and knockout mouse strains for use in experiments. Such genetically altered strains are powerful analytic tools for defining the role of specific gene products in complex biologic systems, and have become the "reagents" that allow fundamental questions to be experimentally addressed that had been previously unapproachable. Mice with induced mutations frequently have to be bred onto other background strains for optimal study, which requires screening of individual animals in each generation by identification of one or more target genetic elements. Dr. Charles Elson will serve as Director and Dr. Casey Weaver will serve as Co-Director. Mice will be housed under barrier conditions in a new vivarium which is physically linked to the new Shelby Research Building. Dr. Sam Gartner from the Department of Comparative Medicine will serve as Consultant An Oversight Committee consisting of the PI of each project with Drs. Schoeb and Gartner will oversee the usage and function.