CORE--ANIMAL MODEL

核心--动物模型

• 批准号: 6959581
• 负责人: CHARLES O ELSON
• 金额: $ 23.11万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6959581
• 关键词: animal breeding; animal colony; colitis; disease /disorder model; gene expression; genetic manipulation; genetic strain; genetically modified animals; laboratory mouse; model design /development; pathologic process; tissue resource /registry

Animal Model will provide all mice used and it will be responsible for ordering mice, performing the various manipulations required to induce experimental colitis, maintaining a barrier facility, performing routine surveys to ensure absence of pathogens in the colony, and assist in harvesting tissues from such mice. Centralization of these functions provides uniformity and quality control of mice being studied in the various projects, reduces cost, and will help conserve numbers of mice required by ensuring their optimal use in the projects. A second aim is that of centralized pathologic analyses which will be performed by Dr. Trenton Schoeb, an expert veterinary pathologist. Tissue sections from the various projects will be coded prior to interpretation and quantitative scoring where needed. This facilitates interactions among the projects and also the comparison of results. A third purpose is to provide genetically modified transgenic and knockout mouse strains for use in experiments. Such genetically altered strains are powerful analytic tools for defining the role of specific gene products in complex biologic systems, and have become the "reagents" that allow fundamental questions to be experimentally addressed that had been previously unapproachable. Mice with induced mutations frequently have to be bred onto other background strains for optimal study, which requires screening of individual animals in each generation by identification of one or more target genetic elements. Dr. Charles Elson will serve as Director and Dr. Casey Weaver will serve as Co-Director. Mice will be housed under barrier conditions in a new vivarium which is physically linked to the new Shelby Research Building. Dr. Sam Gartner from the Department of Comparative Medicine will serve as Consultant An Oversight Committee consisting of the PI of each project with Drs. Schoeb and Gartner will oversee the usage and function.

动物模型将提供所有使用的小鼠，它将负责订购小鼠，执行诱发实验性结肠炎所需的各种操作，维护屏障设施，执行常规检查以确保群体中没有病原体，并协助 从这样的老鼠身上获取组织。这些功能的集中提供了不同项目中正在研究的MICE的一致性和质量控制，降低了成本，并将有助于 通过确保它们在项目中的最佳使用来节省所需的MICE数量。第二个目标是将由特伦顿·舍布博士进行的集中病理分析， 一个专业的兽医病理学家。不同项目的组织切片将在 在需要的地方进行解释和量化评分。这有利于项目之间的互动，也有利于比较结果。第三个目的是提供用于实验的转基因和基因敲除小鼠品系。这些基因改变的菌株是确定特定基因产物在复杂生物系统中作用的强大分析工具，并已成为允许通过实验解决以前无法接近的基本问题的“试剂”。频繁发生诱变的小鼠 必须繁殖到其他背景菌株上进行最佳研究，这需要通过鉴定一个或多个目标遗传元素对每一代中的单个动物进行筛选。查尔斯·埃尔森博士将担任主任，凯西·韦弗博士将担任联席主任。小鼠将被安置在一个新的隔膜条件下，该隔膜与新的谢尔比研究大楼物理上相连。来自比较医学系的萨姆·加特纳博士将担任顾问，一个由每个项目的PI和舍布博士组成的监督委员会将监督该药物的使用和功能。