CORE--ANIMAL MODEL

核心--动物模型

• 批准号: 7486785
• 负责人: CHARLES O ELSON
• 金额: $ 26.27万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7486785
• 关键词: Address; Animal Model; Animals; Breeding; Code; Colitis; Complex; Condition; Ensure; Generations; Housing; Individual; Induced Mutation; Knock-out; Link; Medicine; Modeling; Mouse Strains; Mus; Numbers; Pathologic; Pathologist; Proteins; Purpose; Quality Control; Reagent; Research; Research Personnel; Role; Score; Screening procedure; Surveys; System; Tissue Harvesting; Tissues; Transgenic Organisms; comparative; cost; genetic element; pathogen; programs; research study; tool

Animal Model will provide all mice used and it will be responsible for ordering mice, performing the various manipulations required to induce experimental colitis, maintaining a barrier facility, performing routine surveys to ensure absence of pathogens in the colony, and assist in harvesting tissues from such mice. Centralization of these functions provides uniformity and quality control of mice being studied in the various projects, reduces cost, and will help conserve numbers of mice required by ensuring their optimal use in the projects. A second aim is that of centralized pathologic analyses which will be performed by Dr. Trenton Schoeb, an expert veterinary pathologist. Tissue sections from the various projects will be coded prior to interpretation and quantitative scoring where needed. This facilitates interactions among the projects and also the comparison of results. A third purpose is to provide genetically modified transgenic and knockout mouse strains for use in experiments. Such genetically altered strains are powerful analytic tools for defining the role of specific gene products in complex biologic systems, and have become the "reagents" that allow fundamental questions to be experimentally addressed that had been previously unapproachable. Mice with induced mutations frequently have to be bred onto other background strains for optimal study, which requires screening of individual animals in each generation by identification of one or more target genetic elements. Dr. Charles Elson will serve as Director and Dr. Casey Weaver will serve as Co-Director. Mice will be housed under barrier conditions in a new vivarium which is physically linked to the new Shelby Research Building. Dr. Sam Gartner from the Department of Comparative Medicine will serve as Consultant An Oversight Committee consisting of the PI of each project with Drs. Schoeb and Gartner will oversee the usage and function.

动物模型将提供所有使用的小鼠，并负责对小鼠进行排序，执行诱导实验性结肠炎所需的各种操作，维护屏障设施，进行常规调查以确保群体中不存在病原体，并协助 从这些小鼠身上获取组织。这些功能的集中提供了在各个项目中研究的小鼠的一致性和质量控制，降低了成本，并将有助于 通过确保项目中的最佳使用来节省所需的小鼠数量。第二个目标是由 Trenton Schoeb 博士进行集中病理分析， 兽医病理学家专家。来自各个项目的组织切片将在之前进行编码 需要时进行解释和定量评分。这有利于项目之间的互动以及结果的比较。第三个目的是提供用于实验的转基因和基因敲除小鼠品系。这种基因改变的菌株是定义特定基因产物在复杂生物系统中的作用的强大分析工具，并且已成为允许通过实验解决以前无法解决的基本问题的“试剂”。频繁发生诱导突变的小鼠 为了进行最佳研究，必须将其培育到其他背景品系上，这需要通过鉴定一个或多个目标遗传元件来筛选每一代的个体动物。查尔斯·埃尔森博士将担任主任，凯西·韦弗博士将担任联合主任。小鼠将被安置在一个新的动物饲养场中，该动物饲养场与新的谢尔比研究大楼有物理连接。比较医学系的 Sam Gartner 博士将担任顾问。监督委员会由每个项目的 PI 和 Drs. 组成。 Schoeb 和 Gartner 将监督其使用和功能。