Animal Model Core

动物模型核心

• 批准号: 7992808
• 负责人: CHARLES O ELSON
• 金额: $ 45.63万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7992808
• 关键词: Address; American; Animal Model; Code; Colitis; Ensure; Flagellin; Goals; Housing; Immune response; Inflammatory Bowel Diseases; Instruction; Knockout Mice; Medicine; Modeling; Mouse Strains; Mus; Pathologic; Pathologist; Quality Control; Reporter; Research Personnel; Specific Pathogen Frees; Surveys; T-Cell Receptor; Tissue Harvesting; Tissues; Transgenic Mice; Transgenic Organisms; adaptive immunity; comparative; cost; germ free condition; insight; mouse model; novel; pathogen; programs; research study

PROJECT SUMMARY (See instructions): Animal Model Core B will provide all mice used in Projects 1, 2, and 3. The Core will be responsible for ordering mice, performing various manipulations required to induce experimental colitis, maintaining a specific pathogen-free barrier facility, performing roufine surveys, to ensure absence of pathogens in the colony, and assist in harvesting tissues from mice. Centralization of these functions in an Animal Model Core provides uniformity and quality control of mice being studied in the various projects, reduces cost, and will help conserve numbers of mice required by ensuring their optimal use in the projects. A second aim of the Animal Model Core is that of centralized pathologic analyses which will be performed by Dr. Trenton Schoeb, an expert veterinary pathologist. Tissue sections from the various projects are coded prior to interpretation and quantitative scoring where needed. This facilitates interactions among the projects and also the comparison of results between them. A third purpose of the Animal Model Core is to provide genetically modified transgenic and knockout mouse strains for use in experiments in the various projects. All of the above Aims have been accomplished in the previous cycle. In regard to the third Aim, during the previous cycle a number of novel genetically-modified mouse lines have been generated in Projects 1, 2, and 3 that have been extremely valuable in addressing innate and adaptive immunity to the microbiota, which is the central goal of this Program Project. These mouse lines include a CBirl flagellin T cell receptor transgenic mouse, an IL-10/Thy1.1 reporter mouse (lOBiT), an IL-17F/Thy1.1 reporter mouse, and an IFNy/Thyl.l reporter mouse. These mouse lines will be housed in Core B and made available to the different projects in this renewal application. Dr. Charies Elson will serve as Director and Dr. Casey Weaver will serve as Co- Director in this Core. Dr. Sam Cartner from the Department of Comparative Medicine will serve as Consultant. An Oversight Committee consisting of the PI of each Project plus Drs. Schoeb and Cartner will oversee the usage and function of the Core.

项目总结（见说明）： 动物模型核心B将提供项目1、2和3中使用的所有小鼠。核心将负责 排序小鼠，进行诱导实验性结肠炎所需的各种操作，维持 特定的无病原体屏障设施，进行roufine调查，以确保 集落，并协助收获组织从小鼠。将这些功能集中在动物模型核心中 提供了在各种项目中研究的小鼠的一致性和质量控制，降低了成本， 通过确保项目中老鼠的最佳使用，帮助节省所需的老鼠数量。第二个目标 动物模型核心是由Dr.特伦顿Schoeb进行的集中病理分析， 兽医病理专家在解释之前，对来自不同项目的组织切片进行编码 以及必要时的定量评分。这有助于项目之间的互动， 比较他们之间的结果。动物模型核心的第三个目的是提供基因 改良的转基因和基因敲除小鼠品系，用于各种项目的实验。所有 上述目标已在上一个周期实现。关于第三个目标，在前一个 在项目1、2和3中已经产生了许多新的基因修饰小鼠品系， 在解决对微生物群的先天性和适应性免疫方面非常有价值， 本项目的核心目标。这些小鼠系包括CBirl鞭毛蛋白T细胞受体转基因小鼠。 小鼠、IL-10/Thy1.1报告小鼠（10 BiT）、IL-17 F/Thy1.1报告小鼠和IFN γ/Thy1.1 报道小鼠。这些鼠标线将被安置在核心B中，并提供给不同的项目， 这一更新申请。Charies Elson博士将担任主任，凯西韦弗博士将担任副主任。 导演在这个核心。来自比较医学系的Sam Cartner博士将担任 顾问。由每个项目的PI以及Schoeb博士和Cartner博士组成的监督委员会将 监督核心的使用和功能。