Uropathogen Detection Using DNA Biosensors

使用 DNA 生物传感器检测尿路病原体

• 批准号: 7074588
• 负责人: BERNARD M CHURCHILL
• 金额: $ 184.89万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-15 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7074588
• 关键词: adolescence (12-20); bacterial DNA; bacterial RNA; bioengineering /biomedical engineering; biosensor device; child (0-11); clinical research; diagnosis design /evaluation; diagnostic tests; electrochemistry; human subject; messenger RNA; microarray technology; microfluidics; nucleic acid quantitation /detection; oligonucleotides; ribosomal RNA; spina bifida; urinalysis; urinary tract disorder diagnosis; urinary tract infection; virulence

DESCRIPTION (provided by the applicant): Urinary tract infection is the most common urological disease in the United States and is a major cause of patient morbidity and health-care expenditure. This Bioengineering Research Partnership proposal involves development of a microelectromechanical system for the genotypic detection and species-specific identification of uropathogens within a time frame (5-10 minutes from sample collection to readout) that would enable point-of-care diagnosis and treatment. The focus of this proposal is to produce a self-contained microbial pathogen detection device and to examine its performance using clinical urine samples. Research at UCLA has provided two key technological advances that make development of a uropathogen sensor feasible. The first is microfluidics for sample processing. The second is an electrochemical microsensor which allows ultrasensitive detection of specific DNA-RNA or DNA-DNA hybridization events, without the need for target amplification. This project has been in development for over a year involving a multidisciplinary effort including leaders in the fields of microfluidics and microsensor technology, molecular microbiology, pediatrics and biomathematics. Specific Aim 1 describes how microfluidics studies will be applied to development of a crossflow filter for uropathogen concentration, micromixing for processing of uropathogen nucleic acids, and washing of the sensor surface. Specific Aim 2 involves fabrication of the microsensor array, development of a streptavidin self-assembled monolayer, and testing of oligonucleotide probes for electrochemical detection of uropathogen rRNA and mRNA on the microsensor surface. Specific Aim 3 will involve integration of the microfluidics and sensor components and testing of its analytic validity on simulated and actual urine specimens. Specific Aim 4 will involve batch fabrication of the device and clinical examination of the association between urosensor results and clinical correlates of urinary tract infection.

描述（申请人提供）：泌尿道感染最多

项目成果

Models for estimating bayes factors with applications to phylogeny and tests of monophyly.

用于估计贝叶斯因子的模型及其在系统发育和单系测试中的应用。

• DOI: 10.1111/j.1541-0420.2005.00352.x
• 发表时间: 2005
• 期刊: Biometrics.
• 作者: Suchard,MarcA;Weiss,RobertE;Sinsheimer,JanetS
• 通讯作者: Sinsheimer,JanetS

A Microfluidic Cartridge System for Multiplexed Clinical Analysis.

• DOI: 10.1016/j.jala.2009.05.002
• 发表时间: 2009-12-01
• 期刊: JALA (Charlottesville, Va.)
• 作者: Lawi W;Wiita C;Snyder ST;Wei F;Wong D;Wong PK;Liao JC;Haake D;Gau V
• 通讯作者: Gau V

Electrochemical molecular analysis without nucleic acid amplification.

• DOI: 10.1016/j.ymeth.2005.05.008
• 发表时间: 2005-09
• 期刊: METHODS
• 影响因子: 4.8
• 作者: Gau, V;Ma, SC;Wang, H;Tsukuda, J;Kibler, J;Haake, DA
• 通讯作者: Haake, DA

An unsteady microfluidic T-form mixer perturbed by hydrodynamic pressure.

受流体动压扰动的非稳态微流体 T 形混合器。

• DOI: 10.1088/0960-1317/18/4/045015
• 发表时间: 2008
• 期刊: Journal of micromechanics and microengineering : structures, devices, and systems
• 作者: Ma,Yanbao;Sun,Chien-Pin;Fields,Michael;Li,Yang;Haake,DavidA;Churchill,BernardM;Ho,Chih-Ming
• 通讯作者: Ho,Chih-Ming