Exploring the Untapped Chemical Potential of the Microbial Word - a cellular and synthetic biology approach to organic synthesis

探索微生物世界未开发的化学潜力 - 有机合成的细胞和合成生物学方法

• 批准号: 2672998
• 金额: --
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2672998
• 关键词: Exploring; Untapped; Chemical; Potential; Microbial

This project will examine the chemistry of a variety of microorganisms and use these cells to synthesise a range of target compounds relevant to the pharmaceutical industry. We will focus our attention on microorganisms isolated from extreme environments (e.g. volcanic soil, hydrothermal vents, sub-aquatic regions, and sites of meteor impact - so-called "extremophiles". Using an in-house focussed library of organic compounds, we will assess the functional group transformations that can be achieved by these organisms under a range of reaction conditions. Successful reactions will be optimised using standard microbiology techniques and, where possible, improved further using synthetic biology tools (e.g. metabolic engineering, CRISPR-Cas). Finally, these new biotransformations will be applied to the synthesis of a pharmaceutically-relevant target compound through our existing links with industry.

该项目将检查多种微生物的化学性质，并利用这些细胞合成一系列与制药行业相关的目标化合物。我们将重点关注从极端环境（例如火山土壤、热液喷口、水下区域和流星撞击地点）中分离出的微生物 - 所谓的“极端微生物”。使用内部集中的有机化合物库，我们将评估这些生物体在一系列反应条件下可以实现的官能团转化。将使用标准微生物学来优化成功的反应 技术，并在可能的情况下使用合成生物学工具（例如代谢工程、CRISPR-Cas）进一步改进。最后，这些新的生物转化将通过我们现有的工业联系应用于药物相关目标化合物的合成。