Studying full length mRNAs at single-cell resolution

以单细胞分辨率研究全长 mRNA

• 批准号: 2683691
• 金额: --
• 依托单位: Oxford Brookes University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2683691
• 关键词: Studying; full; length; mRNAs; single

Single-cell methods are revolutionising biology. For instance, the journal Science distinguished the application of these methods to developmental biology as the Breakthrough of the Year 2018. The next decade will see an exponential increase in the development and the application of single-cell analysis methods to all areas of the bioscience, which will have transformative effects. Our project will contribute to implement an important aspect of the single cell revolution.The development of a method that profiles full-length mRNAs at single-cell resolution is an urgent need for the single-cell field. The methods that we will develop will allow the study of processes of alternative splicing, RNA editing and alternative polyadenylation in single cells, revealing their dynamics in processes of cell differentiation, development and disease. Studying these with single-cell resolution is important to reconstruct their dynamics and establish their importance. This will impact the fields of stem cell biology, developmental biology and regenerative medicine, but also a broader community of biosciences researchers working in basic biological processes and models of disease.Furthermore, this project will allow applying ONT long read sequencing technology to a state-of-the-art single-cell transcriptomic approach. ONT is a world leader in this type of sequencing, which is growing very fast in the last few years. Our project therefore will help implementing long read sequencing technologies in the biosciences.Importantly, the development of such a method can also lead to biotechnological applications, and to further optimisation to develop commercial solutions to single-cell transcriptomics using ONT signature sequencing platform. The PhD student, at the end of the process, will have all the training in using the technology and single-cell transcriptomics and will therefore be in an ideal position for further developing this technology.

单细胞方法正在彻底改变生物学。例如，《科学》杂志将这些方法在发育生物学中的应用评为 2018 年度突破。未来十年，单细胞分析方法在生物科学所有领域的发展和应用将呈指数级增长，这将产生变革性的影响。我们的项目将有助于实现单细胞革命的一个重要方面。单细胞领域迫切需要开发一种以单细胞分辨率分析全长 mRNA 的方法。我们将开发的方法将允许研究单细胞中的选择性剪接、RNA 编辑和选择性多腺苷酸化过程，揭示它们在细胞分化、发育和疾病过程中的动态。以单细胞分辨率研究这些对于重建它们的动力学并确定它们的重要性非常重要。这将影响干细胞生物学、发育生物学和再生医学领域，而且还将影响更广泛的从事基础生物过程和疾病模型研究的生物科学研究人员群体。此外，该项目将允许将 ONT 长读长测序技术应用于最先进的单细胞转录组学方法。 ONT 是此类测序领域的世界领先者，在过去几年中增长非常快。因此，我们的项目将有助于在生物科学中实施长读长测序技术。重要的是，这种方法的开发还可以带来生物技术应用，并进一步优化以开发使用 ONT 签名测序平台的单细胞转录组学商业解决方案。博士生在该过程结束时将接受使用该技术和单细胞转录组学的所有培训，因此将处于进一步开发该技术的理想位置。