Using ecophysiology to better predict the uptake of chemicals into fish

淡水的化学成分差异很大，例如H+和钙因地理位置的不同而增加1000倍，它们主要影响影响化学物质吸收的化学形态和鳃和皮肤的特性。这个机械化的项目将填补这一知识空白，使生态风险评估能够根据地区条件进行定制。我们的多学科监督团队在生理学、生态毒理学和分析化学方面提供世界级的专业知识，以支持学生参与这一真正新颖的项目。跨越水生动物鳃/皮肤的跨上皮电位(TEP)影响带电分子的摄取，但受到淡水化学自然变化(如pH、Ca、盐度、DOC)的显著影响(例如，从-35 mV到+10 mV)。TEP如何影响化学物质的吸收代表了生态毒理学中的一个主要知识缺口，并可能解释个别鱼类和不同研究之间在它们的吸收方面存在很大的不一致。学生将发展鱼类和细胞培养的体内和体外生理学实验方面的专业知识，包括测量跨越鳃的跨皮细胞电势(TEP)和一系列物种的化学吸收。我们将提供与分析化学和了解化学形态以及统计和建模方法有关的技能培训。

Freshwater chemistry varies greatly, e.g. H+ and calcium by >1,000-fold depending on location, which majorly influence chemical speciation and properties of gills and skin that affect chemical uptake. This mechanistic project will fill this knowledge gap to allow ecological risk assessment to be tailored to match regional conditions. Our multidisciplinary supervisory team provides world-class expertise in physiology, ecotoxicology, and analytical chemistry to support the student in this truly novel project. The transepithelial electrical potential (TEP) across aquatic animal gills/skin influences uptake of charged molecules, but is dramatically influenced (e.g. from -35 to +10 mV) by natural variability in freshwater chemistry (e.g. pH, Ca, salinity, DOC). How TEP affects chemical uptake represents a major knowledge gap in ecotoxicology and may explain large inconsistencies in their uptake between individual fish and different studies.The student will develop expertise in in vivo and in vitro physiology experimentation with fish and cell cultures, including measuring transepithelial electrical potential (TEP) across gills and chemical uptake in a range of species. We will provide training in skills relevant to analytical chemistry and understanding chemical speciation, and statistical and modelling methods.