Biliary morphogenesis and bile canaliculi integration

胆道形态发生和胆小管整合

• 批准号: 7158034
• 负责人: David M Hoganson
• 金额: $ 4.88万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7158034
• 关键词: bile ducts; bioengineering /biomedical engineering; biomaterial development /preparation; biomaterial evaluation; biotechnology; epithelium; growth factor; laboratory rat; liver; liver cells; male; postdoctoral investigator; swine; tissue engineering; tissue support frame; total artificial organ

DESCRIPTION (provided by applicant): The development of a replacement liver for transplant or as an extracorporeal support device is necessary to address the growing liver transplant waiting list and the limited number of available organs. Development of a tissue engineered liver is an exciting possible solution. Integration of hepatocytes and a bile duct system in a three dimensional scaffold where the natural bile canaliculi connect to a bile duct network to support bile flow has not been developed. This research will utilize a novel tissue engineering scaffold with a vascularized three dimensional scaffold structure with compartments for hepatic parenchyma and a biliary system that has been developed. With this scaffold, the factors regulating biliary epithelial cell growth and the mechanism of integration of a bile ducts and bile canaliculi will be identified. The effect of geometry, scaffold materials and growth factors will be studied. This scaffold for a tissue engineered liver will be tested in in-vitro and in-vivo models utilizing mature hepatocytes, small cell hepatocytes, biliary epithelial cells and non-parenchymal cells as cell sources.

描述（由申请人提供）：需要开发用于移植的替代肝或作为体外支撑装置，以解决不断增长的肝脏移植候补名单和有限数量的可用器官。组织工程肝脏的开发是一种令人兴奋的解决方案。在三维支架中的肝细胞和胆管系统的整合，其中天然胆汁的胆管连接到胆管网络以支持胆汁流。这项研究将利用一种新型的组织工程支架，其具有血管化的三维支架结构，并带有用于肝实质的隔室和已开发的胆道系统。通过这种支架，将鉴定调节胆道上皮细胞生长的因素以及胆管和胆管的整合机理。将研究几何，脚手架材料和生长因子的影响。该组织工程肝脏的支架将在使用成熟的肝细胞，小细胞肝细胞，胆道上皮细胞和非核细胞细胞作为细胞源的体外和体内模型中进行测试。