Pain Coding by TRP Channels in Polymodal Nociceptors

多模式伤害感受器中 TRP 通道的疼痛编码

• 批准号: 7142443
• 负责人: VALERIE J UZZELL
• 金额: $ 4.97万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7142443
• 关键词: calcium indicator; electrodes; electrophysiology; ganglions; genetically modified animals; immunocytochemistry; laboratory mouse; neural plasticity; neurons; nociceptors; pain; postdoctoral investigator; receptor expression; receptor sensitivity; somesthetic sensory cortex; thermoreception; tissue /cell culture; touch; trigeminal nerve

DESCRIPTION (provided by applicant): Evidence suggests that temperature and pain sensation in the somatosensory system may be mediated by Transient Receptor Potential (TRP) channels, but this conclusion remains uncertain. To test this hypothesis, it will be necessary to characterize TRP channel function within a defined cell type of intact neurons within their neural circuit. These experiments will determine which TRP channels, if any, mediate pain responses in polymodal nociceptors (PNs) in an ex vivo mouse trigeminal ganglion preparation. PNs, which respond to a range of painful stimuli, will be identified by their calcium responses to heat, cold, and pinch, and their neural coding of painful stimuli and TRP channel expression will be characterized. To confirm the role of the expressed TRP channels in pain responses, wild type PN responses to heat, cold and pinch will be compared to those of knockout mice lacking specific heat and cold sensitive TRP channels. By determining which TRP channels, if any, mediate pain responses in PNs, these experiments will be a crucial step towards understanding sensory transduction of temperature and pain in the somatosensory system and towards identifying potential molecular targets for alleviating pain.

描述（申请人提供）：有证据表明体感系统中的温度和痛觉可能是由瞬时感受器电位（TRP）通道介导的，但这一结论仍不确定。为了检验这一假设，有必要表征神经回路内完整神经元的定义细胞类型内的 TRP 通道功能。这些实验将确定哪些 TRP 通道（如果有的话）介导离体小鼠三叉神经节制剂中多模式伤害感受器 (PN) 的疼痛反应。对一系列疼痛刺激做出反应的 PN 将通过其对热、冷和捏的钙反应来识别，并且其疼痛刺激的神经编码和 TRP 通道表达将被表征。为了确认表达的 TRP 通道在疼痛反应中的作用，将野生型 PN 对热、冷和捏的反应与缺乏特定热和冷敏感 TRP 通道的基因敲除小鼠的反应进行比较。通过确定哪些 TRP 通道（如果有的话）介导 PN 中的疼痛反应，这些实验将是理解体感系统中温度和疼痛的感觉转导以及识别减轻疼痛的潜在分子靶标的关键一步。