Role of the central nucleus of the amygdala during ethanol-rewarded instrumental tasks

杏仁核中央核在乙醇奖励的仪器任务中的作用

• 批准号: 10679383
• 负责人: Matilde Castro
• 金额: $ 4.77万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10679383
• 关键词: Affect; Alcohol consumption; Alcohols; Amygdaloid structure; Anatomy; Animal Behavior; Animals; Attention; Automobile Driving; Behavior; Behavioral; Cell Nucleus; Chronic; Communication; Consumption; Cues; Data; Dependence; Electrodes; Electrophysiology (science); Ethanol; Event; Food; Glass; Implanted Electrodes; Individual; Intake; Intervention; Intoxication; Learning; Mediating; Medical; Monitor; Motivation; Neurobiology; Neurons; Nutrient; Oral; Organism; Output; Pattern; Procedures; Process; Ramp; Rattus; Research; Research Personnel; Rewards; Role; Satiation; Science; Self Administration; Sodium; Substance Use Disorder; Techniques; Testing; Thirst; Time; Training; Translating; Volatilization; Water; Work; Writing; alcohol research; alcohol reward; alcohol seeking behavior; career; drinking; experience; experimental study; improved; in vivo; motivated behavior; motor behavior; neural; neural correlate; neurophysiology; novel therapeutics; optogenetics; pharmacologic; preference; programs; recruit; reinforcer; response; reward processing; skills

PROJECT SUMMARY The motivation to consume ethanol is a fickle thing. It differs wildly between individuals, but even in one individual, this motivation is not always constant. However, the exact dynamics that translate motivation to consume to the literal act of consumption remain poorly understood. The central nucleus of the amygdala may be an essential piece in this process. Its anatomical connections hint at its potential role in ethanol and food reward consumption, given that it is densely innervated by canonical reward processing centers and itself innervates regions directing motor behaviors and attention. However, the exact mechanisms and firing patterns that drive the central nucleus of the amygdala’s involvement in driving reward consumption remain poorly understood. This proposal makes use of a foundational technique, in vivo electrophysiology, to uncover how the central nucleus of the amygdala electrical activity relates to alcohol seeking and taking. In looking at the activity of this region over the course of a whole instrumental task session, a clearer conclusion about the central nucleus of the amygdala’s role in synthesizing motivation to drive motor behaviors can be obtained. In aim 1, the neural activity pattern in the central nucleus of the amygdala will be monitored over the course of an ethanol self-administration task to understand how activity in this region changes across trials as an animal’s motivation to consume ethanol wanes. For aim 2, the proposed electrophysiology recordings will occur when water-deprived rats perform a choice task that promotes a switch in reward preference from water to ethanol. These planned recordings will elucidate central nucleus of the amygdala activity when there are competing motivations. In aim 3, the effect of dependence on both instrumental tasks will be assessed to understand how the firing in this region following dependence-inducing high concentrations of ethanol. In completing these aims and disseminating the data I find, I will also further my independence and confidence as an independent researcher while also improving my theoretical, technical, and professional skills. Overall, this project aims to understand the central nucleus of the amygdala’s role as a nexus point between reward seeking and reward consumption. This would not only deepen our understanding of this nucleus’s role as a whole, but could potentially contribute to novel therapeutic behavioral and medical interventions.

项目总结 消费乙醇的动机是反复无常的。它在不同的人之间差异很大，但即使是在一个人身上 就个人而言，这种动机并不总是恒定的。然而，将动机转化为 消费对消费的字面行为仍然知之甚少。杏仁核中央核可能 成为这一过程中不可或缺的一部分。它的解剖联系暗示了它在乙醇和食品中的潜在作用 奖励消费，因为它是由规范的奖励处理中心和自身密集支配的 支配运动行为和注意力的区域。然而，确切的机制和激发模式 驱动杏仁核中央核参与驱动奖赏消费的方法仍然很差 明白了。这项提议利用一项基础技术--活体电生理学--来揭示 杏仁核中央核团的电活动与寻酒和饮酒有关。在看着 在整个工具性任务会议的过程中这一区域的活动，关于 杏仁核的中央核在合成驱动运动行为的动机方面的作用可以得到。在……里面 目的1，杏仁核中央核团的神经活动模式将被监测在一个 乙醇自我给药任务，以了解该区域的活动如何在实验中作为动物的 消费乙醇的动力减弱。对于目标2，拟议的电生理记录将在以下情况下进行 缺水的大鼠完成了一项选择任务，促进了奖励偏好从水到乙醇的转变。 这些计划中的记录将阐明当存在竞争时杏仁核的中央核活动 动机。在目标3中，将评估依赖对两种工具性任务的影响，以了解如何 这一区域的起火是在高浓度乙醇引发依赖之后进行的。在完成这些目标时 而传播我发现的数据，我也会进一步增强我作为一个独立人士的独立性和信心 研究人员，同时也提高了我的理论、技术和专业技能。总体而言，该项目旨在 理解杏仁核作为寻求奖赏和奖赏的连接点的作用 消费。这不仅将加深我们对这个核心作为一个整体的作用的理解，而且可以 潜在地有助于新的治疗、行为和医学干预。