Investigation of the Nodavirus lifecycle in vivo

诺达病毒体内生命周期的研究

• 批准号: 7052795
• 负责人: Jason Kenneth Lanman
• 金额: $ 4.88万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7052795
• 关键词: RNA virus; computer simulation; electron microscopy; fluorescence microscopy; life cycle; postdoctoral investigator; protein structure; protein structure function; three dimensional imaging /topography; tomography; virus genetics; virus infection mechanism; virus protein

DESCRIPTION (provided by applicant): Flock House Virus (FHV) is a non-enveloped, icosahedral RNA virus that infects insects. FHV has been thoroughly studied genetically, biophysically, and structurally, which makes it an excellent system for extending these studies to investigate the virus lifecycle (virus entry, disassembly, assembly, and cellular exit) in situ. To study the FHV lifecycle in situ the virus coat protein containing the tetracysteine tag will be labeled specifically with biarsenical fluorophores, which are visible in both the fluorescent and electron microscopes allowing the different images to be correlated. Additionally, in optical pulse-chase experiments the FHV will be successively labeled with different colored biarsenical fluorophores to distinguish older and younger coat protein. To investigate at higher resolution the spatial relationship of FHV within cells, tomographic electron microscope images will be used to produce three-dimensional reconstructions of the virus infected cells.

描述（由申请人提供）：