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Suspension arrays detection/quantification of antibodies

悬浮阵列检测/抗体定量

基本信息

批准号：
6885604
负责人：
Alexey G Zdanovsky
金额：
$ 16.02万
依托单位：
ALPHA UNIVERSE, LLC
依托单位国家：
美国
项目类别：
财政年份：
2005
资助国家：
美国
起止时间：
2005-03-01 至 2006-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6885604
关键词：
Clostridium botulinum antibacterial antibody antibody bioterrorism /chemical warfare chimeric proteins flow cytometry high throughput technology ligands microarray technology neurotoxins peptide chemical synthesis peptide library protein protein interaction protein purification protein quantitation /detection proteomics technology /technique development

项目摘要

DESCRIPTION (provided by applicant): In modern medicine and science there is a growing need for the ability to profile composition of such bodily fluids as serum and saliva. Microarray chip technology has potential for delivering such ability. This technology has already changed pharmaceutical research. So far, however, this technology has not been used extensively in protein-focused studies. The reason for this lies in the substantial diversity of protein properties. This diversity affects performance of protein arrays and, consequently, limits the number of analytes that can be simultaneously interrogated by such arrays. Our objective is to develop a set of interconnected protocols and constructs that will constitute a novel approach to protein array design. This approach will allow achieving more uniform efficiencies of: 1) attachment of probe proteins to the matrix of the array, 2) interaction between individual probes and corresponding proteins from the interrogating sample (analytes), and 3) visualization of individual interacting pairs. To achieve our objective we will combine advancements in the ligand-display technology and techniques for generation of chimera proteins with the emerging platform of particle-based flow cytometric assays. First, we intend to apply the developed approach to address the needs existing in the area of bioterrorism defense. Therefore, during Phase I we will demonstrate the feasibility of the approach by developing an array for detection of anti-botulinum serotype A antibodies. During Phase II, we will broaden the spectrum of antibodies detected by the system. We will incorporate into the array antigenic determinants for such pathogenic agents of concern in the field of bioterrorism as Bacillus anthracis, Clostridium botulinum (serotypes B, C and E), and ricin to create a tool for evaluation of the level of individual protection from potential bioterrorist attacks.

描述（由申请人提供）：在现代医学和科学中，越来越需要能够分析血清和唾液等体液的成分。微阵列芯片技术有潜力提供这种能力。这项技术已经改变了药物研究。然而到目前为止，这项技术尚未广泛应用于以蛋白质为重点的研究中。其原因在于蛋白质特性的巨大多样性。这种多样性影响蛋白质阵列的性能，因此限制了此类阵列可以同时询问的分析物的数量。我们的目标是开发一套相互关联的方案和结构，构成蛋白质阵列设计的新方法。这种方法将实现更均匀的效率：1) 将探针蛋白附着到阵列基质上，2) 各个探针和来自询问样品（分析物）的相应蛋白质之间的相互作用，以及 3) 各个相互作用对的可视化。为了实现我们的目标，我们将把配体展示技术和嵌合蛋白生成技术的进步与基于颗粒的流式细胞术检测的新兴平台相结合。首先，我们打算应用已开发的方法来满足生物恐怖主义防御领域的现有需求。因此，在第一阶段，我们将通过开发用于检测抗肉毒杆菌 A 血清型抗体的阵列来证明该方法的可行性。在第二阶段，我们将拓宽系统检测到的抗体谱。我们将把生物恐怖主义领域关注的炭疽杆菌、肉毒杆菌（血清型 B、C 和 E）和蓖麻毒素等病原体的抗原决定簇纳入阵列中，以创建一个工具来评估个人免受潜在生物恐怖袭击的保护水平。