Novel system for sensitive detection of botulinum neurotoxins

灵敏检测肉毒杆菌神经毒素的新型系统

• 批准号: 8714706
• 负责人: Alexey G Zdanovsky
• 金额: $ 15万
• 依托单位: ALPHA UNIVERSE, LLC
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8714706
• 关键词: Antigens; Area; Bacteriophage M13; Bacteriophages; Binding; Bontoxilysin; CDR1 gene; Capsid; Catalytic Domain; Cells; Complementarity Determining Regions; DNA Restriction Enzymes; Detection; Development; Discrimination; Environment; Enzyme-Linked Immunosorbent Assay; Evolution; Genome; Goals; Immunoglobulins; Libraries; Llama; Location; Luciferases; Methods; Modification; Phase; Physicians; Procedures; Property; Proteins; Randomized; Recruitment Activity; Research; Research Design; Research Methodology; Scientist; Serotyping; Signal Transduction; Site; Societies; Structure; Structure-Activity Relationship; System; Terminator Codon; Time; Work; base; biothreat; detector; interest; novel; polypeptide; prevent; public health relevance; receptor; research study; scaffold; screening; vector

DESCRIPTION (provided by applicant): The existence of terrorist organizations around the world, along with their ability to recruit expertise from physicians and scientists, poses an increasing threat of bioterrorist attack to society. In order to reduce the consequences of such an attack or even prevent it, new systems for detection of biothreat agents are necessary. Currently existing "express" methods suffer from either being too expensive and complicated or lacking sensitivity. In contrast, we propose to develop a new system that will be inexpensive, simple to use, and highly sensitive. This system will be created by combining a novel signal amplification approach, developed in our lab, with advances in directed protein evolution. To prove the viability of our approach, during Phase I, we will develop the first components of this detection system targeted at the receptor- recognizing domain of Botulinum Neurotoxin Serotype A. In Phase II, this approach will be used to develop additional detectors for a universal Botulinum Neurotoxin detection system.

描述（由申请人提供）：世界各地恐怖组织的存在，沿着他们从医生和科学家那里招募专业知识的能力，对社会构成了越来越大的生物恐怖袭击威胁。为了减少这种攻击的后果，甚至防止这种攻击，有必要建立新的生物威胁剂检测系统。目前现有的“快速”方法要么过于昂贵和复杂，要么缺乏灵敏度。相比之下，我们建议开发一种新的系统，将是廉价的，简单易用，高度敏感。该系统将通过结合我们实验室开发的一种新的信号放大方法与定向蛋白质进化的进展来创建。为了证明我们的方法的可行性，在第一阶段，我们将开发该检测系统的第一个组件，该组件靶向肉毒杆菌神经毒素血清型A的受体识别域。在第二阶段，这种方法将用于开发通用肉毒杆菌神经毒素检测系统的其他检测器。