CRYOPRESERVATION OF SEMEN AND SOMATIC CELLS

精液和体细胞的冷冻保存

• 批准号: 7391947
• 负责人: MARK E HASKINS
• 金额: $ 0.67万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391947
• 关键词: CRYOPRESERVATION; SEMEN; SOMATIC; CELLS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Semen from carriers or affected dogs affected with genetic diseases that are known or suspected animal models of human genetic disease is collected, diluted, frozen, and stored for the future use. Somatic cells, usually fibroblasts, are grown in culture, harvested, and frozen in liquid nitrogen for later re-culture and study by a number of methods including enzyme assays and DNA Southern blotting/pcr amplification of specified markers for linkage studies, as well as cytogenetic studies. Feline semen samples are collected, frozen, and stored by Drs. JoGayle Howard and Budda Pukazheathi, Genome Resources Bank, Smithsonian Institution, National Zoological Park, Washington DC. Frozen feline embryos are made and stored by Dr. William Swanson, Cincinnati Zoo, in collaboration with his NCRR grant. We have also provided testes from dogs and cats to Drs. Ralph Brinster and Ina Dobrinski for cross-species germ cell transplantation studies. We store semen and fibroblasts from goats with beta-mannosidosis and mucopolysaccharidosis IIID provided by Dr. Margaret Jones, Michigan State University. These colonies were threatened by Dr. Jones retirement, and the semen and fibroblast resources are being stored in liquid nitrogen.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。收集、稀释、冷冻和储存已知或疑似人类遗传病动物模型的遗传病携带者或受影响犬的精液，以备将来使用。体细胞，通常是成纤维细胞，在培养物中生长，收获，并在液氮中冷冻，用于以后的再培养和通过多种方法进行研究，包括酶测定和DNA Southern印迹/PCR扩增用于连锁研究的特定标记，以及细胞遗传学研究。 猫精液样本由华盛顿国家动物园史密森学会基因组资源库的JoGayle霍华德和Budda Pukazheathi博士采集、冷冻和储存。冷冻猫胚胎是由辛辛那提动物园的威廉·斯旺森博士与他的NCRR基金合作制作和储存的。我们还向Ralph Brinster和Ina Dobrinski博士提供了狗和猫的睾丸，用于跨物种生殖细胞移植研究。我们储存了来自密歇根州立大学玛格丽特·琼斯博士提供的患有β-甘露糖苷酶病和粘多糖沉积病III型的山羊的精液和成纤维细胞。这些殖民地受到琼斯博士退休的威胁，精液和成纤维细胞资源被储存在液氮中。