CANINE AND FELINE RED CELL ANTIGENS

犬和​​猫红细胞抗原

• 批准号: 7391971
• 负责人: URS GIGER
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391971
• 关键词: CANINE; FELINE; RED; CELL; ANTIGENS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Hemolytic transfusion reactions are significant, severe complications in human patients as well as domestic animals receiving blood. We have established a collaboration with Steven Spitalnik at Columbia University in New York to further define blood group systems in dogs and cats. Feline Blood Groups Only one blood group system has been thusfar recognized in cats. This feline AB system consists of three blood types: type A, B, and AB. A-B incompatibilities are responsible for acute hemolytic transfusion reactions and hemolysis of the newborn. Our collaborative studies have well characterized the inheritance biochemical nature and immunologic consequences over the past decade. Furthermore, we have assisted in the development and the evaluation of gel test system to detect accurately the three blood types; initial studies on a prototype are very encouraging. Gel test systems have recently becoming standard methods for blood typing in humans. In the process of examining compatibility of animals receiving blood we recently recognized a new blood group antigen. This antigen is named Mik and appears to occur very commonly, hence this may be a novel high frequency red cell antigen. We have documented the development of naturally occurring alloantibodies against this antigen in animals lacking the antigen. Further investigations are planned to better define the antigen and its involvement in hemolytic transfusion reactions. Canine Blood Groups Canine blood groups have been examined for many decades but the biochemical and molecular bases of the more than a dozen blood group systems have not been defined. This is partially due to the lack of appropriate typing reagents and their apparent unimportance at the time of a first transfusion. Nevertheless we documented the clinical importance of the Dog Erythrocyte Antigen (DEA) 1.1 and have applied the same gel test method with a monoclonal antibody to DEA 1.1 for routine screening of dogs. In the process of these studies we also recognized a Dalmatian with a pan-incompatibility to all blood donors tested. This included 50 dogs from various breeds. However, when testing this Dalmatian to other Dalmatians, 3 of 13 were found to be compatible, suggesting that they also lacked this red cell antigen. Because of the common occurrence of this antigen and its absence in some Dalmatians it was named Dal and may represent a new high frequency red cell antigen. Further investigations are planned to better define the antigen and its involvement in hemolytic transfusion rea

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。溶血性输血反应在人类患者以及接受血液的家畜中是显著的、严重的并发症。我们已经与纽约哥伦比亚大学的Steven Spitalnik建立了合作关系，以进一步定义狗和猫的血型系统。到目前为止，在猫身上只发现了一种血型系统。这种猫的AB系统由三种血型组成：A型、B型和AB型。A-B不相容是导致新生儿急性溶血性输血反应和溶血的原因。在过去的十年里，我们的合作研究已经很好地描述了遗传、生化性质和免疫学后果。此外，我们协助开发和评估凝胶测试系统，以准确检测三种血型；对原型机的初步研究非常令人鼓舞。凝胶测试系统最近已经成为人类血型的标准方法。在检查接受血液的动物的相容性过程中，我们最近发现了一种新的血型抗原。这种抗原被命名为Mik，似乎很常见，因此这可能是一种新的高频红细胞抗原。我们已经记录了在缺乏抗原的动物中自然发生的对抗该抗原的同种抗体的发展。计划进一步研究以更好地确定抗原及其在溶血性输血反应中的作用。犬的血型已经被研究了几十年，但十几种血型系统的生化和分子基础还没有被定义。这部分是由于缺乏适当的分型试剂，而且它们在第一次输血时显然不重要。尽管如此，我们证明了犬红细胞抗原（DEA） 1.1的临床重要性，并应用与DEA 1.1单克隆抗体相同的凝胶试验方法进行犬的常规筛查。在这些研究的过程中，我们也发现了一只达尔马提亚犬与所有被测试的献血者都有泛不相容。其中包括50只不同品种的狗。然而，当将这只斑点狗与其他斑点狗进行测试时，发现13只斑点狗中有3只是相容的，这表明它们也缺乏这种红细胞抗原。由于这种抗原在某些斑点狗中常见而不存在，因此被命名为Dal，可能代表一种新的高频红细胞抗原。计划进一步研究以更好地确定抗原及其在溶血性输血中的作用