NATIONAL RESOURCES FOR APLYSIA

海兔国家资源

• 批准号: 7392004
• 负责人: PATRICK J WALSH
• 金额: $ 54.74万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7392004
• 关键词: NATIONAL; RESOURCES; APLYSIA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal describes the continuation of the National Resource for Aplysia, whose overall goal is to provide consistently high-quality cultured sea hares Aplysia californica (and their cultured red algal food, Gracilaria sp.) to NIH-sponsored researchers. Aplysia californica is an important non-vertebrate (Opisthobranch mollusc) model system for health-related research, primarily in the neurophysiology of behavior and learning. We will continue to produce animals for research, and to conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-projects/specific aims: 1. Production-We anticipate increasing our production of animals by approximately 25% per year during this next phase of funding and will make specimens from all life stages available at a price competitive with fieldcollected specimens. 2. Functional Genomics-We will undertake a cDNA/EST sequencing project of neural and other tissues from different developmental stages (larvae, juveniles, and reproductive and senescent animals), and use these cDNAs to produce microarrays. Following appropriate validation and quality control, these microarrays will be available for at-cost purchase by our user community, and will also be used to examine gene expression changes in our hatchery population during different developmental stages, and after exposure to other experimental conditions including variable temperature, egg-laying hormone administration, etc. All sequence and array experimentation data will be made available through web-based database access. 3. Developmental Neurophysiology- We will continue study of ion current modulation in the bag cells observed to vary with development, genetic background, and between hatchery reared and wild caught animals. Understanding electrophysiological correlates of growth and maturation are important to the Resource because they may affect the interpretation of data by users of the Resource. A correlative study will examine how different growth rates of the animals produce variations in development times for the nervous system changes that culminate in sexual maturity. 4. Animal Health Monitoring-We will continue a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animals at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied. Through this combination of production and basic research, we will improve the model system and extend its usefullness to other areas of research.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。描述（由申请人提供）：本提案描述了国家海兔资源的延续，其总体目标是向 NIH 资助的研究人员提供始终如一的高质量养殖海兔加州海兔（及其养殖红藻食物龙须菜属 sp.）。海兔是一种重要的非脊椎动物（后鳃软体动物）模型系统，用于健康相关研究，主要用于行为和学习的神经生理学研究。我们将继续生产用于研究的动物，并开展旨在探索新模式用途和改善资源的基础研究。有四个子项目/具体目标： 1. 生产——我们预计在下一阶段的资助中每年将动物产量增加约 25%，并将以与现场采集的标本具有竞争力的价格提供所有生命阶段的标本。 2. 功能基因组学-我们将承担不同发育阶段（幼虫、幼体、生殖和衰老动物）的神经和其他组织的cDNA/EST测序项目，并利用这些cDNA生产微阵列。经过适当的验证和质量控制后，这些微阵列将可供我们的用户社区以成本价购买，并且还将用于检查我们孵化场群体在不同发育阶段以及暴露于其他实验条件（包括可变温度、产蛋激素管理等）后的基因表达变化。所有序列和阵列实验数据将通过基于网络的数据库访问提供。 3.发育神经生理学-我们将继续研究袋细胞中的离子电流调节，观察到随发育、遗传背景以及孵化场饲养和野生捕获的动物之间的变化。了解生长和成熟的电生理相关性对于资源很重要，因为它们可能会影响资源用户对数据的解释。一项相关研究将研究动物的不同生长速度如何导致最终性成熟的神经系统变化的发育时间变化。 4. 动物健康监测——我们将继续实施基于水质和动物健康参数筛查的监测计划，以确保快速检测并完整记录孵化场动物任何发育阶段可能发生的任何疾病过程。将调查观察到的任何疾病症状和可疑病原体并采取适当的控制措施。通过生产与基础研究的结合，我们将完善模型系统并将其实用性扩展到其他研究领域。