Development of Analytical Methods for the Analysis of mRNA Therapeutics

该项目旨在开发和优化新型高效液相色谱（HPLC）和质谱分析方法，以开发更快、更可靠、信息更丰富（更高分辨率）的方法来表征RNA和mRNA治疗方法。最初的工作将集中在优化离子对反相液相色谱（IP RP HPLC），结合高温分离分析大RNA和mRNA治疗药物。在项目期间，学生将获得HPLC方法开发和分析，质谱，RNA生物化学和凝胶电泳方面的专业知识。

The aim of the proposed studentship is to develop and optimise novel high performance liquid chromatography (HPLC) and Mass Spectrometry methods for developing faster, robust and more informative (higher resolution) methods for the characterization of RNA and mRNA therapeutics. Initial work will focus on optimising Ion Pair Reverse Phase Liquid Chromatography (IP RP HPLC) in conjunction with high temperature separations for the analysis of large RNA and mRNA therapeutics. During the project the student will gain expertise in HPLC method development and analysis, mass spectrometry, RNA biochemistry and gel electrophoresis.