CORE--BIOPHYSICS AND ELECTROPHYSIOLOGY

核心--生物物理学和电生理学

• 批准号: 7554013
• 负责人: Kathleen Dunlap
• 金额: $ 12.11万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7554013
• 关键词: Xenopus oocyte; biomedical facility; biophysics; cell line; electrophysiology; neurosciences; recombinant proteins; voltage /patch clamp

This Core is not based on an existing Core and represents a novel concept that is likely to increase the research capabilities of a large number of Neuroscience Investigators as well as promote collaborations. The goal of this Core is to provide equipment for electrophysiological experiments as well as the technical capability for performing the experiments. This will allow Neuroscience investigators to carry out preliminary experiments that will test the feasibility of applying electrophysiological approaches to ongoing projects. In addition, the Core will provide a well-defined venue for Neuroscience Investigators to obtain expert advice regarding establishing electrophysiological methods in their own laboratories. This Core will be situated adjacent to the laboratories of the Director and Co-Directors (Dunlap; Blaustein & Cox). A key component of the Core that will be critical for its success is the hiring of an expert electrophysiologist. The equipment available will allow a variety of recording techniques including whole cell current and voltage clamp, single channel recording, two electrode voltage clamp, tissue slice single cell current and voltage clamp and extracellular field recording. As currently proposed, the Core would eventually have 2 rigs, the 2nd one being purchased in the 2nd year and dedicated primarily for slice electrophysiology. While the idea of having an electrophysiology core is well regarded, it is important to have realistic goals for the core. The nuances associated with slice recording that are unique to each preparation raise questions about the feasibility of incorporating slice electrophysiology into the Core. The goals of the 1st rig seem more realistic and feasible to achieve with the hiring of a well trained electrophysiologist.

这个核心不是基于现有的核心，而是一个新的概念，可能会增加大量神经科学研究者的研究能力，并促进合作。该核心的目标是提供电生理学实验设备以及执行实验的技术能力。这将使神经科学研究人员能够进行初步实验，测试将电生理方法应用于正在进行的项目的可行性。此外，核心将为神经科学研究者提供一个明确的场所，以获得有关在自己的实验室建立电生理方法的专家建议。该核心将位于 主任和联合主任（Dunlap; Blaustein &考克斯）的实验室。核心的一个关键组成部分，将是其成功的关键是聘请一位专家电生理学家。可用的设备将允许各种记录技术，包括全细胞电流和电压钳、单通道记录、双电极电压钳、组织切片单细胞电流和电压钳以及胞外场记录。根据目前的提议，Core最终将有2个钻机，第2个钻机将在第2年购买，主要用于切片电生理学。虽然拥有电生理学核心的想法得到了很好的考虑，但重要的是要为核心制定现实的目标。与之相关的细微差别 切片记录是每种制备物所独有的，这引起了关于将切片电生理学并入核心的可行性的问题。第一台钻机的目标似乎更现实和可行的实现与训练有素的电生理学家的雇用。