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Mitochondrial Modulation of Endothelial Phenotype

Mitochondrial Modulation of Endothelial Phenotype
内皮表型的线粒体调节
批准号:
7137141
负责人:
John F. Keaney
金额:
$38.84万
依托单位国家:
美国
项目类别:
财政年份:
2005
资助国家:
美国
项目状态:
已结题
起止时间:
2005-09-30 至 2010-08-31

项目摘要

项目成果

John F. Keaney的其他基金

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中文摘要
翻译
内皮是正常血管稳态的重要组成部分。众所周知,这是正常的
英文摘要
The endothelium is an important component of normal vascular homeostasis. It is known that normal endothelial function is disturbed in the setting of atherosclerosis and its risk factors such as hypercholesterolemia, diabetes, and hypertension. It is likely that multiple mechanisms contribute to impaired endothelial function, however, oxidant stress in the form of reactive oxygen species (ROS) production appear particularly important. It is also now widely appreciated that ROS act as signaling molecules that contribute to the vascular injury response, but in atherosclerosis ROS signaling becomes dysregulated and contributes to endothelial dysfunction. Despite this knowledge, the mechanisms of ROS signaling in the endothelium remain obscure. This proposal is based upon the central hypothesis that mitochondria are an important component of redox-sensitive signaling and, as a consequence, are a key determinant of endothelial cell phenotype. The objective of this application, therefore, is to determine the role of the mitochondrion in modulating endothelial cell phenotype and elucidate any operative mechanisms. To accomplish this goal, we first will undertake a detailed examination of how endothelial cell phenotype modulates mitochondrial functions such as protonmotive force (delta-muH+), mitochondrial ROS, and uncoupling protein expression. Using this knowledge and reagents we have developed, we will then manipulate specific mitochondrial functions (delta-muH+, UCPs) in cultured endothelial cells and determine the implications for endothelial functions known to involve ROS, such as nitric oxide bioactivity, cell proliferation and migration, and adhesion molecule expression. Because cells imperfectly model events in vivo, we will also manipulate mitochondrial function in vivo using UCP-2 null mice and mice we will develop with inducible endothelial cell-specific over-expression of UCP-2. We will then go on to determine the implications of UCP-2 manipulation endothelial phenotype in vivo both in the resting state and in response to stress in the form of arterial injury. Successful completion of these studies will provide mechanistic information on redox-mediated control of endothelial cell phenotype and afford us the necessary insight to design new therapeutic strategies that focus on improving vascular homeostasis in the setting of atherosclerosis and its risk factors.
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CORE--Biomarker
  • 批准号:
    7140911
  • 项目类别:
  • 资助金额:
    $9.32万
  • 财政年份:
    2006
  • 负责人:
    John F. Keaney
  • 依托单位:
Endothelial Redox State & Phenotype in Health & Disease
  • 批准号:
    6960736
  • 项目类别:
  • 资助金额:
    $229.2万
  • 财政年份:
    2005
  • 负责人:
    John F. Keaney
  • 依托单位:
Nox Isoforms and Vascular Cell Phenotype
  • 批准号:
    7172934
  • 项目类别:
  • 资助金额:
    $30.63万
  • 财政年份:
    2005
  • 负责人:
    John F. Keaney
  • 依托单位:
Nox Isoforms and Vascular Cell Phenotype
  • 批准号:
    7014035
  • 项目类别:
  • 资助金额:
    $31.54万
  • 财政年份:
    2005
  • 负责人:
    John F. Keaney
  • 依托单位:
海外基金