Biosensor to measure microscopic cell function

测量微观细胞功能的生物传感器

• 批准号: 7109046
• 负责人: Alan B Moy
• 金额: $ 14.47万
• 依托单位: CELLULAR ENGINEERING TECHNOLOGIES, INC
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2009-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7109046
• 关键词: animal tissue; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biological signal transduction; biomedical equipment development; biosensor device; cell adhesion; cell component structure /function; cell membrane; cell motility; cytotoxicity; electrical measurement; intracellular; molecular /cellular imaging; nanotechnology; optics; photonics; surface property; tissue /cell culture

DESCRIPTION (provided by applicant): This is a revised application. The broad, long-term objectives of this Phase I STTR proposal is to demonstrate feasibility in developing a novel optical-electronic biosensor for microscopic applications to permit simultaneous integration of photonic and electronic signals for comprehensive time-lapsed microscopy applications. Optical microscopy has limitations in sensitivity to evaluate the impact of signal transduction on cell-membrane function. Light microscopy is unable to detect cell-membrane displacement at the low nanometer level, and its specificity is limited by the potential over-interpretation of observed microscopic changes at the micron level. Thus, optical signals need to be properly interpreted within the contexts of an independent method. In contrast, electronic sensors have the resolution to measure cell surface cell membrane function at the low nanometer level but have limited intracellular spatial resolution. In collaboration with Iowa State University, CET Inc. will develop a novel, optical-electronic biosensor that will measure several functional parameters in cultured cells in a nondestructive fashion with a resolution of transmission electron microscopy. This sensor will combine the complementary strengths of electronic and optical signals. The outcome of this research is to demonstrate feasibility that will leverage a Phase II proposal that leads to further developments in sensor design, software and hardware development and image analysis and signal processing to provide comprehensive information on signal transduction in cultured living cells. The commercial product will provide a comprehensive platform for cell microscopists. The specific objectives for Phase I of this STTR are: (1) fabricate an optical-electronic sensor that can quantitatively and dynamically measure cell adhesion and motility without electrode signal degradation; (2) experimentally validate that the sensor is not inherently toxic to cells over both short and long time periods; (3) validate that the sensor does not impede the photonic signals from phase, DIC, and fluorescent microscopy.

描述（由申请人提供）：这是修订的申请。该阶段ISTTR建议的广泛，长期目标是证明可行性用于开发新型的光学电子生物传感器，用于微观应用，以允许同时整合光子和电子信号，以进行全面的时间段的显微镜应用。光学显微镜在敏感性方面具有局限性，以评估信号转导对细胞膜功能的影响。光学显微镜无法在低纳米水平上检测细胞膜位移，并且其特异性受到观察到的微小变化在微米水平的过度解释的限制。因此，需要在独立方法的上下文中正确解释光学信号。相反，电子传感器具有在低纳米水平下测量细胞表面细胞膜功能的分辨率，但细胞内空间分辨率有限。与爱荷华州立大学合作，CET Inc.将开发一种新型的光学电子生物传感器，该传感器将以无损的方式测量培养细胞中的几个功能参数，并分辨出透射电子显微镜。该传感器将结合电子和光学信号的互补强度。这项研究的结果是证明可行性，该可行性将利用II期提案，从而导致传感器设计，软件和硬件开发以及图像分析和信号处理的进一步发展，以提供有关培养的活细胞中信号转导的全面信息。商业产品将为细胞显微镜医生提供全面的平台。该STTR的I期的特定目标是：（1）制造一个光学传感器，可以在没有电极信号降解的情况下定量和动态测量细胞粘附和运动性； （2）实验验证传感器在短期和长时间内对细胞的固有毒性固有毒性； （3）验证传感器不会从相，DIC和荧光显微镜中妨碍光子信号。