Ultrafast SBS Method for Large-Scale Human Resequencing

用于大规模人体重测序的超快 SBS 方法

• 批准号: 7192585
• 负责人: Michael L. Metzker
• 金额: $ 38.4万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-29 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7192585
• 关键词: Ultrafast; SBS; Method; Large; Scale

DESCRIPTION (provided by applicant): Over the past six months, we have made substantial progress towards development of biologically-active reversible terminators. The discovery of a new class of modified nucleosides has established the foundation for development of a four-color reversible terminator set in the near future. Still, tremendous work is required in organic synthesis, compound modeling, and UV deprotection to move the technology from the stage of feasibility to practical application. We are requesting supplemental funding to accelerate our progress for developing the ultra-fast DNA sequencing strategy. Here, we present our recent progress on the development of our first dye-labeled, reversible terminator and step-wise sequencing through a homopolymer repeat. Additional research goals are proposed to deliver a four-color, reversible terminator nucleotide set within the next 12-18 months for implementation of the cyclic reversible termination (CRT) sequencing platform.

描述(申请人提供)：在过去的六个月里，我们在开发具有生物活性的可逆终止剂方面取得了实质性进展。新型修饰核苷的发现为在不久的将来开发四色可逆终止器奠定了基础。然而，要将这项技术从可行性阶段转移到实际应用阶段，还需要在有机合成、化合物建模和紫外线防护方面做大量的工作。我们正在申请补充资金，以加快我们开发超快DNA测序战略的进度。在这里，我们介绍了我们在开发第一个染料标记的可逆终止子和通过均聚物重复进行逐步测序方面的最新进展。其他研究目标是在未来12-18个月内提供四种颜色的可逆终止子核苷酸集，用于实施循环可逆终止(CRT)测序平台。