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Efficient Creation of Long-Template Libraries for Next-Generation Sequencing

高效创建用于下一代测序的长模板库

基本信息

批准号：
9049170
负责人：
Michael L. Metzker
金额：
$ 14.68万
依托单位：
REDVAULT BIOSCIENCES, LP
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-01-14 至 2017-02-13
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9049170
关键词：
Address Agriculture Amaze Area Biological Sciences Chemistry Clinical Communities Complex Couples Custom DNA DNA Library DNA Sequence Data Development Diagnostic Disadvantaged Disease Enzymes Forensic Medicine Foundations Generations Genome Genomic DNA Genomic medicine Genomics Glean Health History of Medicine Human Hybridization Array Investigation Lead Length Libraries Methodology Methods Microfluidics Natural History New England Nucleic Acids Nucleotides Phase Play Population Dynamics Preparation Process Productivity Protein Array Protein Hybridization RNA RNA-Protein Interaction Reading Reagent Replicon Research Research Personnel Role Running Sampling Solid Structure Surface Systems Biology Technology Variant comparative genomics cost density design genome analysis genome-wide human DNA sequencing improved innovation innovative technologies method development next generation sequencing public health relevance rapid detection reference genome screening sequencing platform small molecule structural genomics technology development tool tumor progression tumorigenesis wasting

项目摘要

DESCRIPTION (provided by applicant): The advent of next-generation sequencing (NGS) technologies has allowed researchers to make unprecedented progress in the analysis of genomes, the results of which have profound implications in the realms of human health, agricultural productivity, and (natural history). The ability to fully characterize genomes remains unrealized however due in large part to our inability to reconcile sequence information over long distances. Short fragmented DNA libraries and their subsequent alignment to reference genomes, while highly useful in identifying single nucleotide variants (SNV), make de novo genome assembly and genomic structural analysis difficult. Long template NGS sequencing offers a potential solution but suffers from complex and expensive library preparation strategies. Such strategies frequently require significant amounts of input DNA due to complicated and inefficient manipulation steps. We have proposed an approach for creating long template libraries that addresses these issues with a simple, efficient, and relatively inexpensive method involving the innovative pairing of long dumbbell templates with solid-phase rolling circle replication. The successful development of this technology would greatly enhance the research community's ability to interrogate genomes, particularly those with large structural variation which likely plays a significant role in oncogenesis and tumor progression.