How do we make and process the RNA involved in DNA repair

我们如何制造和加工参与 DNA 修复的 RNA

• 批准号: 2741361
• 金额: --
• 依托单位: University of Sheffield
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2741361
• 关键词: How; do; make; process; RNA

DNA Double strand breaks (DSBs) elicit bidirectional transcription in mammalian cells. The transcribed RNA is processed by DROSHA to generate short RNAs and RNA is required for recruitment of DNA repair factors. HNRNPUL1 and HNRNPUL2 are RNA/DNA binding proteins which associate with each other and are required for DSB repair by an unknown mechanism (Mol Cell v45 p505). We have discovered that HNRNPUL1 binds RNA polymerase II (POLII) and is required for efficient transcription at sites of DSBs and other RNAs processed by DROSHA. HNRNPUL2 binds the 5' CAP binding complex, which orchestrates RNA processing (NAR v48 p10456). Therefore, we hypothesise that HNRNPUL1 drives transcription and HNRNPUL2 the processing of RNAs produced at DSBs. This project will focus on the role of HNRNPUL2 in this process.Objectives: Establish the molecular role HNRNPUL2 plays in production/processing of RNA at DSBs and its role in recruitment of DNA repair factors.

DNA双链断裂(DSB)诱导哺乳动物细胞双向转录。转录的RNA由DROSHA处理以产生短RNA，而RNA是招募DNA修复因子所必需的。HNRNPUL1和HNRNPUL2是相互结合的RNA/DNA结合蛋白，是DSB修复所必需的未知机制(Mol Cell V45 P505)。我们已经发现HNRNPUL1与RNA聚合酶II(POLII)结合，并且是在DROSHA处理的DSB和其他RNA的位点高效转录所必需的。HNRNPUL2与5‘CAP结合复合体结合，后者协调RNA处理(NAR V48 P10456)。因此，我们假设HNRNPUL1驱动转录，HNRNPUL2驱动DSB产生的RNA的加工。本项目将重点研究HNRNPUL2在这一过程中的作用。目的：建立HNRNPUL2在DSB的RNA生产/加工中的分子作用及其在DNA修复因子招募中的作用。