Core--Organic Synthesis - Synthesis of Lipid Mediators

化学核心将通过正在研究的特定新型脂质介体的合成和分析，为该研究计划提供必要的化学支持。这一核心将负责所需数量(最多毫克规模)的一组生物活性脂氧素、解脂素、二十二碳三烯、二磷酸前烯衍生物和相关脂质介体的化学合成，这些物质将用于研究它们在解决与牙周病相关的炎症中的作用。化学核心将开发和优化所需的合成途径，以立体化学纯形式制备选定的脂类介体，并将对其进行全合成、详细提纯和结构验证。 化学核心还将在目标脂质介体的鉴定、结构阐明和研究方面与其他子项目密切合作，并将帮助制备用于受体结合研究的同位素标记的衍生物。总体而言，化学核心的工作对于研究本计划研究的脂类介体的关键作用，阐明它们在涉及解析的信号通路中的作用，以及建立它们潜在的“促消解”和抗炎作用是至关重要的。

The Chemistry Core will provide essential chemistry support for this Research Program through the synthesis and analysis of the specific novel lipid mediators being studied. This Core will be responsible for the chemical synthesis of the required quantities (up to milligram-scale) of a panel of bioactive lipoxins, resolvins, docosatrienes, presqualene diphosphate derivatives and related lipid mediators that will be used to study their role in the resolution of inflammation associated with periodontal disease. The Chemistry Core will develop and optimize the required synthetic pathways for the preparation of the selected lipid mediators in a stereochemically pure form and will carry out their total synthesis, detailed purification and structural verification. The Chemistry Core will also collaborate closely with the other Sub-Projects on the identification, structural elucidation and study of the targeted lipid mediators and will help prepare isotopically labeled derivatives for receptor binding studies. Overall, the work performed in the Chemistry Core is essential for studying the key actions of the lipid mediators studied in this Program, for elucidating the their role in the signaling pathways involved in resolution, and for establishing their potential "pro-resolution" and anti-inflammatory roles.