Membrane Mechanisms of Olfactory Transduction

嗅觉传导的膜机制

• 批准号: 6976743
• 负责人: FRANK ZUFALL
• 金额: $ 29.36万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-04-01 至 2006-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6976743
• 关键词: Membrane; Mechanisms; Olfactory; Transduction

EXCEED THE SPACE PROVIDED. This application is for renewal of research directed toward understanding the detection, discrimination, and transduction of sensory information by neurons of the vomeronasal organ (VNO). There has been significant advancement on the principles of signal transduction and odor detection/discrimination by olfactory receptor neurons (ORNs) in the main olfactory epithelium (MOE), but surprisingly little is known about the functional properties of sensory neurons in the VNO. The main goal of this project is to overcome this critical gap between MOE and VNO studies by investigating response properties of mouse vomeronasal neurons (VNs) and examine the cellular and molecular mechanisms that mediate chemosensory detection in these cells. To reach this goal, experiments in this application will take advantage of newly developed mouse VNO preparations by which the functional analysis of pheromone-induced activity in VNs using electrophysiological and high resolution optical imaging techniques can be integrated with the analysis of the pharmacological and molecular properties of these cells. As a result, it is now possible to define the stimulus range, also known as 'receptive field', that can be detected by the mouse VNO and to conduct functional mapping studies that are designed to reveal the basic organization of pheromone detection in this tissue. Other experiments will determine the mechanisms that underlie the detection of chemical signals in VNs and dissect the steps mediating pheromone transduction in these neurons. Taken together, this research will significantly advance our understanding of the mechanisms that underlie pheromone recognition and discrimination at the first stage of the mouse accessory olfactory system and fill critical gaps in our current knowledge. These experiments are not only fundamentally important to pheromone coding and the organization of the sense of smell, but also to understanding principles of chemical communication regulating sexual, hormonal, and reproductive function in mammals.

超过提供的空间。 该应用是用于续签用于理解检测的研究， vomeronasal器官（VNO）神经元（VNO）神经元的歧视和转导感官信息。 信号转导和气味的原理已经取得了重大进步 主要嗅觉上皮（MOE）中嗅觉受体神经元（ORN）的检测/歧视 但令人惊讶的是，关于VNO中感觉神经元的功能特性知之甚少。这 该项目的主要目标是通过研究MOE和VNO研究之间的这一关键差距 小鼠呕吐神经元（VNS）的反应特性，并检查细胞和分子 这些细胞中介导化学感应检测的机制。为了实现这一目标，对此进行实验 应用程序将利用新开发的鼠标VNO准备功能 使用电生理学和高分辨率光学分析信息素酮诱导的VNS活性 成像技术可以与药理和分子特性的分析集成 这些细胞。结果，现在可以定义刺激范围，也称为“接受” 字段'，可以通过小鼠vno检测到，并进行功能映射研究 旨在揭示该组织中信息素检测的基本组织。其他实验将 确定在VN中检测化学信号的基础的机制并剖析步骤 介导这些神经元中的信息素转导。综上所述，这项研究将大大 促进我们对信息素识别和歧视基础的机制的理解 鼠标附件嗅觉系统的第一阶段，并在我们当前的知识中填补关键空白。 这些实验不仅对信息素编码和组织至根本重要 嗅觉，同时也了解化学通信的原则，以调节性别， 哺乳动物中的激素和生殖功能。