Odor recognition in the olfactory epithelium

嗅觉上皮细胞的气味识别

• 批准号: 6597578
• 负责人: FRANK ZUFALL
• 金额: $ 22.85万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6597578
• 关键词: chemoreceptors; electrophysiology; genetically modified animals; laboratory mouse; olfactions; receptor expression; respiratory epithelium; tissue /cell preparation; voltage /patch clamp

Electrophysiology and calcium imaging will be used to study olfactory receptor neuron (ORN) responsiveness in a novel tissue slice preparation. Three specific aims are delineated. In the first specific aim, the sensitivity, selectivity and adaptive properties of the ORNs will be studied using loose, perforated and whole cell patch recording. In the second aim, imaging of intracellular calcium will be used to determine whether odors elicit stereotypic patterns of neuronal activity in the epithelium, and whether these patterns correlate with the olfactory receptor expression zones of the olfactory epithelium. Finally, in the third specific aim experiments are proposed to study responsiveness of ORN expressing a specific receptor (P2) in a gene-targeted mouse co-expressing the marker protein taulacZ.

电生理学和钙成像将用于研究 新组织切片中的嗅觉受体神经元（ORN）反应性 准备。划定了三个具体目标。在第一个特定目标中 将研究ORN的灵敏度，选择性和适应性特性 使用松动，穿孔和全细胞贴片记录。在第二个目标中 细胞内钙的成像将用于确定气味是否引起 上皮中神经元活性的刻板印象模式，以及这些是否是否 模式与嗅觉的嗅觉受体表达区有关 上皮。最后，提出了第三个特定目标实验来研究 ORN表达特定受体（P2）的反应性 小鼠共表达标记蛋白taulacz。