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Bacterial cell envelope remodelling in Rhizobium leguminosarum: contribution to symbiosis and resistance to abiotic stress

豆根瘤菌的细菌细胞包膜重塑：对共生和抵抗非生物胁迫的贡献

基本信息

批准号：
2744522
负责人：
金额：
--
依托单位：
University of Sheffield
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2022
资助国家：
英国
起止时间：
2022 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2744522
关键词：
Bacterial cell envelope remodelling Rhizobium

项目摘要

The aim of this project is to investigate how bacterial cellenvelope remodelling by Ldts contributes to symbiosis (inpea and bean) and to resistance to abiotic stress (heat,osmotic pressure, or acidic conditions). We will alsoinvestigate peptidoglycan structure and dynamics acrossrhizobium isolates to understand how cell envelopecomposition impacts persistence and fitness in the soil.This project will lay the foundation to engineer Rhizobiumstrains for sustainable agriculture.This multidisciplinary project involves 3 academicsupervisors with complementary expertise, based inSheffield (where most of the experimental work will becarried out), York and Oxford. The student will benefit froma training in biochemistry (HPLC and mass spectrometryfor peptidoglycan structural analysis, recombinant proteinexpression, enzymatic assays), bacterial genetics(construction of mutants), comparative genomics andrhizobium-plant interactions. The student will also spend 4months abroad working with the industrial partner (ProteinMetrics, California) to contribute to the development ofsoftware tools dedicated to "peptidoglycomics" analyses.Overall, the student will acquire a comprehensive set ofskills to explore mechanistic insights at molecular andcellular level in bacteria, and in the context of theinteraction with the plant.

该项目的目的是研究Ldts对细菌细胞膜的重塑如何促进共生（在豌豆和豆类中）和抵抗非生物胁迫（热、渗透压或酸性条件）。我们还将研究根瘤菌分离物的肽聚糖结构和动力学，以了解细胞包膜组成如何影响土壤中的持久性和适应性。该项目将为可持续农业的根瘤菌工程奠定基础。这个多学科项目涉及3名学术主管，他们的专业知识互补，分别位于谢菲尔德（大部分实验工作将在这里进行）、约克和牛津。学生将受益于生物化学（高效液相色谱法和质谱法用于肽聚糖结构分析，重组蛋白表达，酶分析），细菌遗传学（突变体构建），比较基因组学和根瘤菌与植物相互作用的培训。该学生还将在国外与工业合作伙伴（ProteinMetrics, California）一起工作4个月，为“肽糖组学”分析的软件工具开发做出贡献。总体而言，学生将获得一套全面的技能，以探索细菌分子和细胞水平的机制见解，并在与植物相互作用的背景下。