CORE E: PROTEOMICS CORE

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。具体目标#1。招聘和雇用一名副主任的蛋白质组学核心设施。博士学位将招募一名具有蛋白质质谱分析经验的高级科学家，负责管理和运营蛋白质组学核心设施。P.I.之间的讨论和项目主任的建议，卡哈尔研究所确定生物学院的兴趣，聘请一名高级教师专门从事蛋白质组学，以加强蛋白质组学核心设施的运作。学院一致投票赞成这一提议。一个搜索委员会将在今年夏天成立，招聘工作将于2005年秋季开始。 具体目标#4购买激光捕获显微切割设备。激光捕获显微切割设备将用于分析组织内选定细胞中的蛋白质。设备演示安排做激光捕获显微切割显微镜的动手评估。Leica Instruments和Molecular Machines举办了为期一天的研讨会。生物系的所有教师和研究生都受到了邀请。约35名教师和学生参加了讲习班。选择购买的仪器将在下一个赠款财政年度初完成。 具体目标#5购买辅助蛋白质组学设备。将获得用于高分辨率蛋白质分离的二维凝胶电泳和蛋白质斑点切除的设备。 通过与Bio-Rad和GE（阿默舍姆）销售代表的讨论，对点切割器设备进行了评价。选择并订购了Bio-Rad EXQuest Spot Cutter，沿着成像设备和图像分析软件。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Specific aim #1. Recruit and hire an associate director for the Proteomics Core facility. A Ph.D. level scientist with experience in protein mass spectrometry will be recruited and hired to supervise and operate the Proteomics Core Facility. Discussions between the P.I. and the Program Director resulted in a proposal that the Cajal Institute determine the Biology Faculty interest in hiring a senior level faculty member specializing in proteomics to enhance the operation of the Proteomics Core Facility. The Faculty unanimously voted in favor of this proposal. A search committee will be formed over the summer, with recruiting beginning in Fall, 2005. Specific aim #4. Purchase laser capture microdissection equipment. Laser capture microdissection equipment will be obtained for analyzing proteins in selected cells within a tissue. Equipment demonstrations were arranged to do hands-on evaluation of laser capture microdissection microscopes. One-day workshops were presented by Leica Instruments and by Molecular Machines. All faculty and graduate students in the Biology Department were invited. The workshops were attended by about 35 faculty and students. Selection of an instrument to purchase will be completed early in the next grant fiscal year. Specific aim #5. Purchase accessory proteomics equipment. Equipment for two-dimensional gel electrophoresis and protein spot excision will be obtained for high resolution protein separations. Spot cutter equipment was evaluated through discussions with Bio-Rad and GE (Amersham) sales representatives. A Bio-Rad EXQuest Spot Cutter was selected and ordered, along with imaging equipment and image analysis software.