EFFECTS OF MERCURIC CHLORIDE ON MALE REPRODUCTION

氯化汞对男性生殖的影响

• 批准号: 7336054
• 负责人: HARI Om GOYAL
• 金额: $ 1.22万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7336054
• 关键词: EFFECTS; MERCURIC; CHLORIDE; MALE; REPRODUCTION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this study was to determine effects of mercuric chloride (MC) treatment on sperm functions (number, motility, morphology), and to determine whether the MC-induced lower fertility resulted from adverse effects on males, females, or both. Both male and female rats were treated with MC at a dose of 2.5 mg/kg or de-ionized water (controls) daily, from 1-90 days of age in the case of males or from 1-111 days of age in the case of females (20 additional days of lactation). Results revealed that mercuric chloride exposure resulted in i) reduced weight of the testis, epididymis, and prostate; ii) reduced number of sperm in the testis and epididymis; iii) increased incidence of abnormal sperm in semen; iv) reduced percentage of motile sperm and progressively forward moving sperm; v) lower concentration of 17 beta estradiol, but without any alteration in serum testosterone or LH level; and vi) decreased fertility index by almost 20%. The reduction in fertility index probably resulted from toxic effects of mercury on sperm parameters, although the relative contribution of each sperm parameter (for example, number, morphology, or motility) in lowering male fertility remains to be determined. Similarly, mercuric chloride exposure lowered fertility index in females. But, compared to males, the reduction in fertility was much higher (reduced by almost 40% vs 20% in males), despite without any reduction in the weight of the ovary and uterus. Hence, these results suggest a higher toxic effect of mercuric chloride exposure on female fertility. In conclusion, results provide evidence for toxic effects of mercury on reproductive organs and fertility in males and on fertility in females, but the mechanism of lower fertility remains unclear.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。本研究的目的是确定氯化汞（MC）处理对精子功能（数量、活力、形态）的影响，并确定MC诱导的生育力降低是否是由于对雄性、雌性或两者的不良影响。从1-90日龄（雄性）或1-111日龄（雌性）（额外20天哺乳期）开始，每天用2.5 mg/kg剂量的MC或去离子水（对照）处理雄性和雌性大鼠。结果表明，氯化汞暴露导致：（一）睾丸、附睾和前列腺重量减少;（二）睾丸和附睾中精子数量减少;（三）精液中异常精子的发生率增加;（四）活动精子和前向运动精子的百分比减少;（五）17 β雌二醇浓度降低，但血清睾酮或LH水平无任何变化;和vi）使生育指数降低近20%。生育力指数的降低可能是由于汞对精子参数的毒性作用，尽管每个精子参数（例如，数量、形态或活力）在降低男性生育力方面的相对贡献仍有待确定。同样，氯化汞暴露降低了女性的生育指数。但是，与男性相比，生育能力的降低要高得多（降低了近40%，男性为20%），尽管卵巢和子宫的重量没有任何减少。因此，这些结果表明，氯化汞暴露对女性生育力的毒性作用更大。总之，研究结果为汞对男性生殖器官和生育力以及女性生育力的毒性效应提供了证据，但生育力降低的机制仍不清楚。