THE MOLECULAR MAGNETIC RESONANCE (MMR) CORE FACILITY

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。最近在NMR实验的设计和执行以及NMR数据的分析和解释方面的突破，创造了确定新类别的生物大分子（包括较大的蛋白质和大分子复合物）的溶液结构和行为的可能性。作为一种结构工具，NMR是X射线晶体学的有益补充。然而，当必须在溶液中研究分子时，它就变得不可或缺了。此外，现在正在开发的NMR技术，可以描述，在原子细节，动态过程，如蛋白质和RNA折叠，蛋白质-蛋白质和蛋白质-核酸复合物的形成，结合诱导的构象变化。另一方面，电子顺磁共振（EPR）已成为研究顺磁性分子磁性的首选方法。原则上，这两种方法可以互补地用于解决同一组问题，因为这两种技术都是基于分子的磁性。能够阐明生物大分子结构特性的磁共振实验特别需要配备有最先进附件的可靠、维护良好的磁共振光谱仪（NMR和EPR）。一个良好的MMR设施将大大促进杰克逊州立大学RCMI项目更新申请中描述的四个子项目中的三个子项目的成功完成。此外，JSU的MBRS/SCORE计划的三个当前和三个预期的主题需要经常使用这个核心设施。因此，MMR核心设施的长期目标是通过提供可靠的现代磁共振光谱仪和高质量的技术服务，加强和提高教师发展和专业培训的机会，这是JSU RCMI计划的总体目标。该项目的具体目标是：（1）为所有在研究中需要复杂结构测定的研究人员提供最先进的仪器;（2）为所有需要使用磁共振能量但不擅长操作仪器的用户提供熟练的技术支持;（3）保持所有光谱仪的最佳技术规格，并保证其可用于研究需要;以及（4）通过提供额外的附件（例如新探头、可变温度单元、梯度单元等）来扩展设施的当前能力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent breakthroughs in the design and execution of NMR experiments and in the analysis and interpretation of NMR data have created the possibility of determining the solution structure and behavior of new categories of biological macromolecules, including larger proteins and macromolecular complexes. As a structural tool, NMR is a useful complement to X-ray crystallography. However, it becomes indispensable when molecules have to be studied in solution. Moreover, NMR techniques are now being developed that can describe, in atomic detail, dynamic processes such as protein and RNA folding, protein-protein and protein-nucleic acid complex formation, and binding-induced conformational changes. On the other hand, electron paramagnetic resonance (EPR) has been the methods of choice for studying the magnetic properties of paramagnetic molecules. In principle, the two methods can be used complementarily to address the same set of problems as both techniques are based on the magnetic properties of the molecule. Magnetic resonance experiments capable of elucidating the structural properties of biological macromolecules have a particular need for reliable, well maintained magnetic resonance spectrometers (NMR and EPR) equipped with the state-of-the-arts accessories. A good MMR facility will dramatically facilitate the successful completion of three out of the four subprojects described in the renewal application of the RCMI program at Jackson State University. In addition, three current and three anticipated subjects of JSU¿s MBRS/SCORE program need to frequently use this core facility. Therefore, the long term objective of the MMR core facility is to strengthen and enhance opportunities for faculty development and professional training, the overall goal of the RCMI program at JSU, by providing reliable modern magnetic resonance spectrometers and high quality technical services. The specific aims of this project are: (1) to provide the state-of-the-arts instrumentation for all investigators who need sophisticated structural determinations in their research; (2) to offer proficient technical support for all users who need to use the power of magnetic resonance but are not experts in operating the instruments; (3) to maintain all spectrometers in their optimum technical specifications and guarantee their availability for research needs; and (4) to expand the facility's current capability by providing additional accessories, such as new probes, variable temperature unit, gradient unit etc.