THE MOLECULAR MAGNETIC RESONANCE (MMR) CORE FACILITY

分子磁共振 (MMR) 核心设施

• 批准号: 7336102
• 负责人: KEN LEE
• 金额: $ 9.5万
• 依托单位: JACKSON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7336102
• 关键词: MOLECULAR; MAGNETIC; RESONANCE; MMR; CORE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Recent breakthroughs in the design and execution of NMR experiments and in the analysis and interpretation of NMR data have created the possibility of determining the solution structure and behavior of new categories of biological macromolecules, including larger proteins and macromolecular complexes. As a structural tool, NMR is a useful complement to X-ray crystallography. However, it becomes indispensable when molecules have to be studied in solution. Moreover, NMR techniques are now being developed that can describe, in atomic detail, dynamic processes such as protein and RNA folding, protein-protein and protein-nucleic acid complex formation, and binding-induced conformational changes. On the other hand, electron paramagnetic resonance (EPR) has been the methods of choice for studying the magnetic properties of paramagnetic molecules. In principle, the two methods can be used complementarily to address the same set of problems as both techniques are based on the magnetic properties of the molecule. Magnetic resonance experiments capable of elucidating the structural properties of biological macromolecules have a particular need for reliable, well maintained magnetic resonance spectrometers (NMR and EPR) equipped with the state-of-the-arts accessories. A good MMR facility will dramatically facilitate the successful completion of three out of the four subprojects described in the renewal application of the RCMI program at Jackson State University. In addition, three current and three anticipated subjects of JSU¿s MBRS/SCORE program need to frequently use this core facility. Therefore, the long term objective of the MMR core facility is to strengthen and enhance opportunities for faculty development and professional training, the overall goal of the RCMI program at JSU, by providing reliable modern magnetic resonance spectrometers and high quality technical services. The specific aims of this project are: (1) to provide the state-of-the-arts instrumentation for all investigators who need sophisticated structural determinations in their research; (2) to offer proficient technical support for all users who need to use the power of magnetic resonance but are not experts in operating the instruments; (3) to maintain all spectrometers in their optimum technical specifications and guarantee their availability for research needs; and (4) to expand the facility's current capability by providing additional accessories, such as new probes, variable temperature unit, gradient unit etc.

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。最近在设计和执行核磁共振实验以及分析和解释核磁共振数据方面的突破创造了确定新类别生物大分子的溶液结构和行为的可能性，包括更大的蛋白质和大分子复合体。作为一种结构工具，核磁共振是对X射线结晶学的有益补充。然而，当分子必须在溶液中研究时，它就变得不可或缺。此外，目前正在开发的核磁共振技术可以详细地描述动态过程，如蛋白质和RNA折叠，蛋白质-蛋白质和蛋白质-核酸复合体的形成，以及结合诱导的构象变化。另一方面，电子顺磁共振(EPR)已成为研究顺磁分子磁性的首选方法。原则上，这两种方法可以互补使用来解决同一组问题，因为两种技术都是基于分子的磁性。能够阐明生物大分子结构性质的磁共振实验特别需要配备最先进附件的可靠、维护良好的磁共振光谱仪(核磁共振和EPR)。一个好的MMR设施将极大地促进杰克逊州立大学RCMI项目续签申请中描述的四个子项目中的三个项目的成功完成。此外，联合州立大学S MBRS/SCORE项目的三个当前科目和三个预期科目需要经常使用这一核心设施。因此，MMR核心设施的长期目标是通过提供可靠的现代磁共振波谱仪和高质量的技术服务，加强和增加教师发展和专业培训的机会，这也是JSU RCMI项目的总体目标。该项目的具体目标是：(1)为在研究中需要复杂结构确定的所有研究人员提供最先进的仪器；(2)为所有需要使用磁共振力量但不是操作仪器的专家的用户提供熟练的技术支持；(3)保持所有光谱仪的最佳技术规格，并保证它们可用于研究需要；以及(4)通过提供更多附件，如新的探头、变温装置、梯度装置等，扩大设施的现有能力。