The Second Wave of Neurogenesis in the DRG

DRG 中的第二波神经发生

• 批准号: 7055571
• 负责人: LYNN D GEORGE
• 金额: $ 4.88万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7055571
• 关键词: neurogenesis; neurons

DESCRIPTION (provided by applicant): The mature dorsal root ganglion (DRG) is a heterogeneous mixture of ca 20 different classes of sensory neurons in addition to glial cells. The mechanisms by which this diverse array of cell types derives from the neural crest remains incompletely understood. There are two waves of neurogenesis in the DRG. Progenitors for the first wave are migrating neural crest cells that stop along their ventral migration pathway, coalesce, and differentiate into proprioceptive and mechanoceptive neurons. The second wave composed primarily of nociceptive afferents that are TrkA+, are bom after the cessation of neural crest migration. Initially TrkA+ neurons are dispersed throughout the nascent DRG, but within 3 days they become dramatically confined to the dorsal medial 2/3 of the ganglion. The mechanisms mediating this spatial segregation are not at all understood. Furthermore, the progenitor population that gives rise to the 2nd wave remains ill defined. The goals of this proposal are to identify and characterize the progenitor cells that give rise to neurons born during the 2nd wave of neurogenesis, and to image, via time-lapse confocal microscopy, the birth and behavior of nociceptive neurons as they differentiate within the maturing DRG.

描述（由申请人提供）：成熟的背根神经节（DRG）是除神经胶质细胞外约 20 种不同类别的感觉神经元的异质混合物。神经嵴衍生出多种细胞类型的机制仍不完全清楚。 DRG 中有两波神经发生。第一波的祖细胞是迁移的神经嵴细胞，它们沿着腹侧迁移路径停止、合并并分化为本体感觉和机械感觉神经元。第二波主要由 TrkA+ 伤害性传入神经组成，在神经嵴迁移停止后产生。最初，TrkA+ 神经元分散在整个新生的 DRG 中，但在 3 天内，它们明显局限于神经节的背侧内侧 2/3。调节这种空间隔离的机制尚不清楚。此外，引起第二波浪潮的祖先群体仍然不明确。该提案的目标是识别和表征在第二波神经发生期间产生神经元的祖细胞，并通过延时共聚焦显微镜对伤害性神经元在成熟的 DRG 中分化时的产生和行为进行成像。