The Second Wave of Neurogenesis in the DRG

DRG 中的第二波神经发生

• 批准号: 7339849
• 负责人: LYNN D GEORGE
• 金额: $ 5.2万
• 依托单位: MONTANA STATE UNIVERSITY - BOZEMAN
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7339849
• 关键词: Address; Afferent Neurons; Behavior; Birth; Caliber; Cells; Class; Confocal Microscopy; Data; Dorsal; Embryo; Fellowship; Ganglia; Goals; Green Fluorescent Proteins; Image; Individual; Label; Lateral; Life; Location; Measurement; Medial; Mediating; Myxoid cyst; Names; Neural Crest; Neural Crest Cell; Neuroepithelial; Neuroepithelial Cells; Neuroglia; Neurons; Nociception; Nociceptors; Pathway interactions; Population; Reporter; Retroviridae; Route; Source; Spinal Cord; Spinal Ganglia; Staging; Stem cells; Techniques; Time; cell type; daughter cell; day; immunocytochemistry; migration; neurogenesis; notch protein; progenitor; segregation; spinal nerve posterior root; time use

The mature dorsal root ganglion (DRG) is a heterogeneous mixture of ca 20 different classes of sensory neurons in addition to glial cells. The mechanisms by which this diverse array of cell types derives from the neural crest remains incompletely understood. There are two waves of neurogenesis in the DRG. Progenitors for the first wave are migrating neural crest cells that stop along their ventral migration pathway, coalesce, and differentiate into proprioceptive and mechanoceptive neurons. The second wave, composed primarily of nociceptive afferents that are TrkA+, are bom after the cessation of neural crest migration. Initially TrkA+ neurons are dispersed throughout the nascent DRG, but within 3 days they become dramatically confined to the dorsal medial 2/3 of the ganglion. The mechanisms mediating this spatial segregation are not at all understood. Furthermore, the progenitor population that gives rise to the 2nd wave remains ill defined. The goals of this proposal are to identify and characterize the progenitor cells that give rise to neurons born during the 2nd wave of neurogenesis, and to image, via time-lapse confocal microscopy, the birth and behavior of nociceptive neurons as they differentiate within the maturing DRG.

成熟的背根神经节（DRG）是由大约20种不同类型的感觉神经元组成的异质混合物。 除了神经胶质细胞。这些不同的细胞类型来源于 神经嵴仍然不完全了解。在DRG中有两个神经发生波。 第一波的祖先是迁移的神经嵴细胞，它们沿着它们的腹侧迁移路径停止， 合并并分化成本体感受神经元和机械感受神经元。第二波，由 主要是TrkA+的伤害性传入，在神经嵴迁移停止后产生。 最初，TrkA+神经元分散在整个新生DRG中，但在3天内，它们变成 显著局限于神经节的背内侧2/3。调节这种空间的机制 隔离是完全不被理解的。此外，引起第二波的祖先人口 仍然不明确。该提案的目标是识别和表征祖细胞， 在神经发生的第二波期间产生神经元，并通过延时共聚焦成像 在显微镜下，伤害感受神经元在成熟的DRG内分化时的出生和行为。