Single cell mass spectrometry

单细胞质谱分析

• 批准号: 2744830
• 金额: --
• 依托单位: University of Surrey
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2744830
• 关键词: Single; cell; mass; spectrometry

This research project aims to improve the robustness of single cell sampling and subsequent mass spectrometry analysis to determine cellular penetration of drug molecules and their effect on cellular biology. This will extend the application of the Live Single Cell Mass Spectrometry technology currently used at GSK and the University of Surrey to demonstrate drug is within target cells (drug at target) to characterising on and off-target effects. The main focus of the project will be on improving robustness of all aspects of the analytical processes, including the use of machine learning for selecting cells based on response and morphological changes.

本研究项目旨在提高单细胞采样和随后的质谱分析的稳健性，以确定药物分子的细胞渗透及其对细胞生物学的影响。这将扩展GSK和萨里大学目前使用的Live单细胞质谱学技术的应用，以证明药物在靶细胞内(靶向药物)，以表征靶上和靶外效应。该项目的主要重点将是提高分析过程所有方面的稳健性，包括利用机器学习根据反应和形态变化选择细胞。