Satellite Glial and Sensory Coding: Implications-Pain

卫星胶质细胞和感觉编码：影响-疼痛

• 批准号: 7016035
• 负责人: ROBERT H LA MOTTE
• 金额: $ 22.07万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-10 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7016035
• 关键词: afferent nerve; calcium flux; calcium indicator; cell cell interaction; electrophysiology; glia; hyperalgesia; imaging /visualization /scanning; laboratory rat; molecular /cellular imaging; nerve injury; neuroimaging; neuronal transport; neurons; pain; potassium channel; soma; spinal ganglion; voltage /patch clamp

DESCRIPTION (provided by applicant): The cell body (soma) of the primary sensory neuron and initial axonal segment are enveloped by a sheath that consists of discrete satellite glial cells each with its own plasma membrane. We know little of the physiological properties of the glia in the dorsal root ganglion and what signals they send and receive. By analogy with astrocytes, it is possible that satellite glia engage in bi-directional ionic and chemical communication with the neurons they ensheathe and with non-neuronal cells. A major question is whether satellite glia have different functional properties according to whether their neurons have nociceptive or non-nociceptive properties. Another question is whether abnormal properties of satellite glia play a role in neuropathic pain by contributing to neuronal hyperexcitability and ectopic discharges originating in the ganglion after trauma or inflammation. With this R21 we explore the novel application of electrophysiology and imaging to the study of the physiological properties of the satellite glia of functionally identified sensory neurons in the rat. We will carry out patch-clamp electrophysiological recording (in vitro), and imaging of calcium signals (in vivo), in satellite glia of visualized neuronal somata. Using intracellular recording from the neuronal soma, we will link the properties of the neuron with the properties of the attached glia and search for bi-directional communication between the two cell types. In addition, we will explore the possibility that these glial properties and possible glial-neuronal interactions are significantly altered after a chronic compression of the dorsal root ganglion (CCD model of neuropathic pain) - an injury known to enhance the excitability of neuronal cell bodies and to produce allodynia and hyperalgesia. The R21 is intended to generate preliminary data and new hypotheses on the physiological properties of satellite glia and their relationships with their sensory neurons in the context of normal and neuropathic mechanisms of pain.

描述（由申请人提供）：初级感觉神经元的细胞体（索马）和起始轴突节段被鞘包被，鞘由离散的卫星胶质细胞组成，每个卫星胶质细胞具有自己的质膜。我们对背根神经节中神经胶质的生理特性以及它们发送和接收的信号知之甚少。与星形胶质细胞类似，卫星胶质细胞可能与它们包裹的神经元和非神经元细胞进行双向离子和化学通讯。一个主要的问题是，卫星胶质细胞是否有不同的功能特性，根据是否有伤害性或非伤害性的神经元的属性。另一个问题是卫星神经胶质细胞的异常特性是否通过促进创伤或炎症后神经元的过度兴奋和起源于神经节的异位放电而在神经病理性疼痛中发挥作用。有了这个R21，我们探索新的应用电生理和成像的生理特性的卫星胶质细胞的功能鉴定的感觉神经元在大鼠的研究。我们将进行膜片钳电生理记录（体外），钙信号成像（体内），在卫星胶质细胞的可视化神经元胞体。利用神经元索马胞体的细胞内记录，我们将把神经元的特性与附着的胶质细胞的特性联系起来，并寻找两种细胞类型之间的双向通讯。此外，我们将探讨的可能性，这些神经胶质细胞的特性和可能的神经胶质细胞-神经元的相互作用显着改变后，慢性压迫的背根神经节（CCD模型的神经病理性疼痛）-损伤已知，以提高神经元细胞体的兴奋性，并产生异常性疼痛和痛觉过敏。R21的目的是产生初步的数据和新的假设的生理特性的卫星神经胶质细胞和它们的关系与他们的感觉神经元的背景下正常和神经病理性机制的疼痛。