Integrated control of Fusarium wilt of lettuce in relation to pathogen and microbial community dynamics

生菜枯萎病与病原菌和微生物群落动态的综合防治

• 批准号: 2746270
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2746270
• 关键词: Integrated; control; Fusarium; wilt; lettuce

Background Fusarium wilt disease of lettuce, caused by the soilborne fungal pathogen Fusarium oxysporum f.sp. lactucae (FOL), is a major constraint to production of protected and field-grown crops globally. Disease resistant lettuce cultivars have been bred to reduce losses due to FOL, but new pathogen races have evolved to overcome this resistance. The most important races are FOL race 1 which is widespread in field lettuce and FOL race 4 which has emerged only very recently to particularly affect protected lettuce in N. Europe. Dual resistance to both races is now being developed, but other approaches for management of FOL as well as an understanding of its biology and dynamics are urgently required to enable a swift response to new outbreaks in a changing climate. The project is a unique collaboration between G's, the University of Warwick and NIAB Cambridge.Objectives and approaches The main aim of this PhD is to develop sustainable integrated disease management approaches for both FOL1 and FOL4 in lettuce with a focus on field production in Spain. The objectives are: i) characterisation of FOL isolates from Spanish lettuce using molecular approaches to establish race identity and potential genetic variation, ii) evaluation of solarisation, biological control and the use of disease resistant / tolerant lettuce varieties as potential components of an integrated control strategy iii) development and implementation of different next generation sequencing approaches for microbial community analyses with a focus on monitoring the dynamics of FOL and beneficial microbes.

背景莴苣枯萎病是由土传真菌病原体尖孢镰刀菌（Fusarium oxysporum f.sp. lactucae，FOL）引起的，是全球保护地和大田作物生产的主要限制因素。已经培育了抗病莴苣品种以减少由于FOL造成的损失，但是新的病原体小种已经进化以克服这种抗性。最重要的小种是FOL小种1和FOL小种4，FOL小种1在田间莴苣中广泛分布，FOL小种4最近才出现，特别影响N.欧洲目前正在开发对两个种族的双重抗性，但迫切需要管理FOL的其他方法以及对其生物学和动力学的了解，以便在不断变化的气候中对新的疫情做出快速反应。该项目是一个独特的G之间的合作，沃里克大学和NIAB剑桥。目标和方法这个博士学位的主要目的是开发可持续的综合疾病管理方法的FOL 1和FOL 4在生菜，重点是在西班牙的现场生产。目标是：i）使用分子方法表征来自西班牙莴苣的FOL分离物以建立种族身份和潜在的遗传变异，生物防治和使用抗病/耐病莴苣品种作为综合防治战略的潜在组成部分iii）开发和实施不同的下一代测序方法，用于微生物群落分析，重点是监测动态FOL和有益微生物。