Comparative Analysis of Cell Death Regulation in Mosquitoes

蚊子细胞死亡调控的比较分析

• 批准号: 7141246
• 负责人: Lei Zhou
• 金额: $ 18.19万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7141246
• 关键词: Anopheles; Drosophilidae; cell death; genes; genome

DESCRIPTION (provided by applicant): Infectious diseases transmitted through mosquitoes account for millions of death world wide each year. Cell death (apoptosis) is an important component of host/vector immuno-response mechanism and has been implicated in the life cycle of pathogens in mosquitoes. However the understanding of cell death regulation in mosquito was hindered greatly by the fact that major insect cell death regulators, namely the reaper/hid/grim (RHG) like lAP-antagonists identified in Drosophila, were not present in the annotated mosquito (Anopheles gambia) genome. Using a systematic bioinformatics approach, we have identified several candidates of reaper/hid/grim-like lAP-antagonist in both the Anopheles and Aedes mosquito genomes. One of them, michelob_x has been characterized as indeed an lAP-antagonist. This proposed project aims at further characterizing these lAP-antagonists and their role in mediating cell death during mosquito development as well as in response to pathogen infection. Findings generated from this study should significantly improve our understanding of immuno-response and cell death regulation in mosquitoes. It is also quite possible that some of these genes, because of their tissue-ablation activity, can be used for mosquito population control strategies.

描述（由申请人提供）：每年通过蚊子传播的传染病占全球数百万死亡。细胞死亡（凋亡）是宿主/载体免疫反应机制的重要组成部分，并且已经与蚊子中病原体的生命周期有关。然而，由于在果蝇（Drosophila）中发现的主要昆虫细胞死亡调节剂（即在果蝇中鉴定出的Lap-Antagonists）中的主要昆虫细胞死亡调节剂（即收割机/HID/GRIM（RHG）），对蚊子中细胞死亡调节的理解受到了极大的阻碍。使用系统的生物信息学方法，我们已经确定了在肛门和艾edes蚊子基因组中的几种收割/HID/Grim样束抗抗逆抗激素的候选者。其中之一，Michelob_x被描述为确实是圈式抗逆饰师。该提出的项目旨在进一步表征这些单抗抗逆抗邦主义者及其在蚊子发育过程中介导细胞死亡以及对病原体感染的反应中的作用。这项研究产生的结果应显着提高我们对蚊子中免疫反应和细胞死亡调节的理解。这些基因中的某些基因的组织活性也很有可能用于蚊子人群控制策略。