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Ultrastructural insights into avian herpesvirus-host cell interactions

禽类疱疹病毒与宿主细胞相互作用的超微结构见解

基本信息

批准号：
2748412
负责人：
金额：
--
依托单位：
University of Oxford
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2022
资助国家：
英国
起止时间：
2022 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2748412
关键词：
Ultrastructural insights into avian herpesvirus

项目摘要

Marek's disease virus (MDV) is a major avian alphaherpesvirus associated with Marek's disease (MD) in poultry characterised by rapid-onset T-cell lymphomas, immunosuppression and paralysis causing annual economic losses of around $2 billion worldwide. Compared to other herpesviruses, MDV has several distinct characteristics, the most important one being its strict cell-associated replication and cell-to-cell spread.This collaborative project between eBIC, the Structural Biology Department at Oxford and the Pirbright Institute aims to gain structural insights into the strict cell-associated lifestyle of this major avian viral pathogen using state-of-the-art tools such as cryogenic electron microscopy (Cryo-EM), electron tomography (Cryo-ET) and focused ion beam (Cryo-FIB) milling at Diamond and Oxford.Project will also aim to explore virus-host interactions using the virology expertise at Pirbright, particularly focusing on the interactions of the major viral oncoprotein MEQ that is associated with the induction of lymphomas and increasing virulence. Thus, the project provides the opportunity to study biogenesis of a cell-associated highly oncogenic herpesvirus as well as explore ultrastructure of viral and cellular protein complexes using the most advanced structural biology tools.

马立克氏病病毒(MDV)是一种主要的禽类甲型疱疹病毒，与家禽马立克氏病(MD)有关，以快速发病的T细胞淋巴瘤、免疫抑制和麻痹为特征，每年造成全球约20亿美元的经济损失。与其他疱疹病毒相比，MDV具有几个明显的特征，其中最重要的是其严格的细胞相关复制和细胞间传播。这个由eBIC、牛津大学结构生物系和皮尔布赖特研究所合作的项目旨在利用最先进的工具，如低温电子显微镜(Cryo-EM)、电子断层扫描(Cryo-ET)和聚焦离子束(Cryo-FIB)在钻石和牛津大学的研磨，从结构上深入了解这种主要禽类病毒病原体严格的细胞相关生活方式。该项目还将致力于利用皮尔布赖特的病毒学专业知识探索病毒与宿主的相互作用。特别关注与淋巴瘤的诱发和毒力增加有关的主要病毒癌蛋白MEQ的相互作用。因此，该项目为研究细胞相关的高度致癌疱疹病毒的生物发生以及利用最先进的结构生物学工具探索病毒和细胞蛋白复合体的超微结构提供了机会。