Large Scale Sequencing and Analysis of Genomes

描述：(申请人提供)本提案的目标是： 为科学界提供数量空前的组装的高质量基因组序列，在数量和质量上足以解决基因组学中的一些核心智力问题。特别是，基因组序列将允许进行比较研究，以确定人类基因组中编码的绝大多数功能元件。它还将允许对动物和真菌王国的生物多样性进行广泛的表征。 我们建议在三年内采取下列措施，以达到这个目标： 鸟枪序列：大约3.31亿个读数，大约205 GB高质量的原始碱基，大约32 GB的高质量草稿基因组组装； 打磨序列：大约10 GB；以及 完成的序列：大约1.5 GB。 我们还计划积极提高基因组测序过程的效率。一个典型哺乳动物基因组的高质量草稿序列(约2.7 GB)的成本在第一年将约为2500万美元，到第三年将降至约1600万美元。

DESCRIPTION: (provided by applicant) The goal of this proposal is: To provide the scientific community with an unprecedented amount of assembled high-quality genome sequence, sufficient in quantity and quality to solve some of the central intellectual questions in genomics. In particular, the genome sequence will allow comparative studies to identify the vast majority of functional elements encoded in the human genome. It will also allow extensive characterization of the biological diversity in the animal and fungal kingdoms. We propose to do this, over a three-year period, by producing: Shotgun sequence: approximately 331 million reads yielding, approximately 205 Gb of high-quality raw bases, and approximately 32 Gb of high-quality draft genome assembly; Polished sequence: approximately 10 Gb; and Finished sequence: approximately 1.5 Gb. We also plan to aggressively improve the efficiency of the genome sequencing process. The cost of high quality draft sequence of a typical mammalian genome (about 2.7 Gb) will be approximately $25M in Year 1 and decrease to about $16M by Year 3.