SEQUENCING THE MOUSE GENOME

怀特黑德/麻省理工学院基因组研究中心渴望为2003年完成小鼠基因组序列的草图和2005年完成的目标做出贡献。在为期三年的项目中，我们计划产生大约25%的小鼠基因组序列覆盖率。具体地说，我们将测序BAC克隆，总计750 Mb-由630 Mb的草稿序列（约5%/碱基）和120 Mb的成品序列（约12%/碱基）组成。 将根据小鼠群落的生物学意义和小鼠-人比较研究选择测序区域。在项目过程中，我们还建议（i）测试涉及BAC克隆池的基因组测序策略，以及（ii）与新的试点中心开展技术转让活动。

The Whitehead/MIT Center for Genome Research is eager to contribute to the goals of completing the sequence of the mouse genome in draft form by 2003 and in finished form by 2005. During the three-year project, we propose to produce sequence coverage of approximately 25 percent of the mouse genome. Specifically, we will sequence BAC clones totaling 750 Mb-consisting of 630 Mb of draft sequence at approximately 5 cent per base and 120 Mb of finished sequence at approximately 12 cent per base. Regions will be selected for sequencing on the bases of biological interest for the mouse community and for comparative mouse-human studies. In the course of the project, we also propose to (i) test strategies for genomic sequencing involving pooling of BAC clones and (ii) engage in technology transfer activities with new pilot centers.