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Development of a field biosensor to monitor fungal activity in situ

开发现场生物传感器来监测原位真菌活动

基本信息

批准号：
2749274
负责人：
金额：
--
依托单位：
University of Strathclyde
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2022
资助国家：
英国
起止时间：
2022 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2749274
关键词：
Development field biosensor monitor fungal

项目摘要

This PhD project will focus on the development of a biosensor which can be used to monitor fungal growth both in large-scale laboratory tests and in field trials in order to understand how fungal biomass evolves over time and space in response to different growth strategies and environmental conditions.This PhD project is part of a larger research programme led by Dr Grainne El Mountassir (UKRI Future Leaders Fellow) which will investigate methods of engineering fungal growth to enhance the stability of soil slopes. A critical aspect in using nature-based solutions to reinforce soil slopes is the ability to monitor the effectiveness of the treatment strategy in situ. This PhD project will focus on the development of an on-the-go, low-cost sensor that can be used for the identification of key biomarkers of the presence of fungi, such as ergosterol (a sterol found in the cell membranes of fungi), chitin ((the major component of fungal cell walls) and hydrophobins (proteins produced by filamentous fungi). The key project aims are to:- Investigate and innovate a detection strategy for key fungal biomarkers using electrochemical biosensors.- Develop a prototype biosensor, characterise and validate the sensor response.- Apply the biosensor to assess the influence of different conditions on the spatial distribution of fungal growth and evolution over time.

该博士项目将专注于开发一种生物传感器，该生物传感器可用于在大规模实验室测试和田间试验中监测真菌生长，以了解真菌生物量如何随时间和空间的变化而变化，以响应不同的生长策略和环境条件。该博士项目是Grainne El Mountassir博士领导的一个更大研究计划的一部分（UKRI未来领袖研究员），将研究工程真菌生长的方法，以提高土壤边坡的稳定性。使用基于自然的解决方案来加固土质边坡的一个关键方面是能够原位监测处理策略的有效性。该博士项目将专注于开发一种可用于识别真菌存在的关键生物标志物的低成本传感器，如麦角固醇（真菌细胞膜中发现的固醇），几丁质（真菌细胞壁的主要成分）和疏水蛋白（丝状真菌产生的蛋白质）。该项目的主要目标是：-使用电化学生物传感器研究和创新关键真菌生物标志物的检测策略。-开发一个原型生物传感器，验证和验证传感器的响应。应用生物传感器评估不同条件对真菌生长和进化随时间的空间分布的影响。