Evolution of Sex Determination in Sticklebacks

刺鱼性别决定的进化

• 批准号: 7094189
• 负责人: Catherine L. Peichel
• 金额: $ 31.99万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7094189
• 关键词: artificial chromosomes; biochemical evolution; cytogenetics; developmental genetics; fish; fluorescent in situ hybridization; gene mutation; genetic library; genetic screening; genetically modified animals; nonmammalian vertebrate embryology; sex chromosomes; sex determination; single strand conformation polymorphism; tissue /cell culture

DESCRIPTION (provided by applicant): Sex determination results from the translation of an initial switch into a complex phenotype that integrates morphology, physiology and behavior to define an organism as male or female. Despite the fundamental importance of this pathway, a wide range of both environmental and genetic sex determination mechanisms is found in nature. Closely related species may have different sex chromosome complements and use completely different mechanisms to determine sex, suggesting that these developmental pathways can evolve very rapidly. How can the rapid evolution of sex determination mechanisms and sex chromosomes occur? Stickleback fish provide an exciting system to address these questions. Recently diverged stickleback species have different sex chromosome complements and may also use different sex-determination mechanisms. Powerful genetic and genomic tools, which make it possible to clone and identify the genes underlying sex determination pathways, have been developed for sticklebacks. This goals of this proposal are to: (1) characterize the sex-determination region found on an evolving sex chromosome in threespine sticklebacks (Gasterosteus aculeatus) through genetic, cytogenetic and physical mapping of the region; (2) molecularly identify the master sex-determination gene in threespine sticklebacks through both loss-of-function and gain-of-function approaches; and (3) compare the chromosomal locations of the master sex-determination gene in closely related species of sticklebacks to determine if sex-determination hierarchies and sex chromosomes have evolved independently in these species. These studies are initial steps towards a long-term goal of comparing sex determination pathways in closely related species to explore the evolution of this fundamental developmental pathway and to determine its effects on the morphology, physiology, and behavior of the organism.

描述（由申请人提供）：性别决定是由初始转换转化为复杂表型的结果，该表型整合了形态学、生理学和行为，以将生物体定义为雄性或雌性。尽管这一途径至关重要，但在自然界中发现了广泛的环境和遗传性别决定机制。密切相关的物种可能有不同的性染色体互补，并使用完全不同的机制来确定性别，这表明这些发育途径可以非常迅速地进化。性别决定机制和性染色体的快速进化是如何发生的？棘背鱼提供了一个令人兴奋的系统来解决这些问题。最近分歧棘鱼物种有不同的性染色体互补，也可能使用不同的性别决定机制。强大的遗传和基因组工具，使克隆和鉴定性别决定途径的基因成为可能，已经为刺鱼开发了。本研究的目标是：（1）研究三刺鱼进化中的性染色体上的性别决定区域（2）通过功能缺失和功能获得两种方法对三刺鱼性别决定主基因进行分子鉴定;以及（3）比较性别决定主基因在刺鱼近缘物种中的染色体位置，以确定性别决定等级和性染色体是否在这些物种中独立进化。这些研究是朝着比较密切相关物种的性别决定途径的长期目标迈出的第一步，以探索这一基本发育途径的演变，并确定其对生物体的形态，生理和行为的影响。