Molecular mechanisms underlying the optimised circadian clock control of Crassulacean acid metabolism

景天酸代谢优化生物钟控制的分子机制

• 批准号: 2749868
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2749868
• 关键词: Molecular; mechanisms; underlying; optimised; circadian

The world is getting hotter and drier due to climate change, and the human population is growing rapidly to the extent that it has been predicted that we will need to increase crop yields by 50 - 70 % by 2050 in order to feed the predicted 9 - 10 billion people. This extra food production has to be achieved using the same land and the same or less fresh water relative to the water used by agriculture today. Achieving such dramatic advances in crop productivity to underpin human food security this century is widely regarded as a key global grand challenge that requires ground-breaking, innovative approaches that "think outside the box". Our research aims to leverage a naturally occurring super-charged adaptation of photosynthesis called Crassulacean acid metabolism (CAM). This adaptation can enhance plant water use efficiency well beyond that of any of today's major food crop species such as rice, wheat or maize, and has, perhaps, even greater utility for feedstock crops for bioenergy and renewable platform chemicals. Through decoding the genomes and transcriptomes of model CAM species in the genus Kalanchoë and undertaking functional genomics research to investigate the function of candidate CAM genes, our work is establishing the minimal parts list for engineering CAM into C3 crops to enhance water use efficiency and photosynthesis. This project will leverage our recent discoveries by exploring the genes involved in CAM using transgenic approaches to switch genes off or on, and/ or explore gene regulation using reporter gene constructs. In particular, we seek to understand how the endogenous circadian clock (the internal timekeeper that organisms use to optimise their biochemistry relative to the daily light/ dark cycle) signals to the CAM system in order to optimize the steps that occur separately both in the dark and the light. This PhD will allow the student to make a key contribution to our understanding of the genetic elements associated with CAM and its optimal temporal regulation. The student will also become accomplished in plant transformation and the techniques required for the detailed molecular, biochemical and physiological characterisation of the generated transgenic lines.The world is getting hotter and drier due to climate change and the human population is growing rapidly to the extent that it has been predicted that we will need to increase crop yields by 50 - 70 % by 2050 in order to feed the predicted 9 - 10 billion people. Our research aims to leverage a naturally occurring super-charged adaptation of photosynthesis called Crassulacean acid metabolism (CAM). This adaptation can enhance plant water use efficiency well beyond that of any of today's major food crop species such as rice, wheat or maize. Through decoding the genomes and transcriptomes of model CAM species in the genus Kalanchoë and undertaking functional genomics research to investigate the function of candidate CAM genes, our work is establishing the minimal parts list for engineering CAM into C3 crops to enhance water use efficiency and photosynthesis. This project will leverage our recent discoveries by exploring the genes involved in CAM using transgenic approaches to switch genes off or on, and/ or explore gene regulation using reporter gene constructs. In particular, we seek to understand how the endogenous circadian clock (the internal timekeeper that organisms use to optimise their biochemistry relative to the daily light/ dark cycle) signals to the CAM system in order to optimize the steps that occur separately both in the dark and the light.

由于气候变化，世界正变得越来越热、越来越干燥，而且人口正在迅速增长，据预测，到2050年，我们需要将粮食产量提高50%至70%，才能养活预计的90亿至100亿人口。与今天的农业用水相比，这种额外的粮食生产必须使用相同的土地和相同或更少的淡水。在作物生产力方面取得如此巨大的进步，以支撑本世纪的人类粮食安全，被广泛认为是一项关键的全球重大挑战，需要突破性的、创新的方法，“跳出框框思考”。我们的研究旨在利用一种自然发生的超强光合作用适应，称为天冬酸代谢（CAM）。这种适应可以提高植物的水分利用效率，远远超过当今任何主要粮食作物物种，如水稻、小麦或玉米，并且可能对生物能源和可再生平台化学品的原料作物有更大的效用。通过对Kalanchoë属CAM模型物种的基因组和转录组进行解码，并开展功能基因组学研究，研究CAM候选基因的功能，建立CAM在C3作物中工程化的最小部件清单，以提高作物的水分利用效率和光合作用。该项目将利用我们最近的发现，通过使用转基因方法来关闭或打开与CAM相关的基因，和/或使用报告基因构建来探索基因调控。特别是，我们试图了解内源性生物钟（生物体用来优化其生物化学相对于每日光/暗周期的内部计时器）如何向CAM系统发出信号，以优化在黑暗和光明中分别发生的步骤。这个博士学位将使学生对我们对与CAM相关的遗传因素及其最佳时间调节的理解做出关键贡献。学生还将完成植物转化和所需的技术详细的分子，生化和生理特性的产生转基因系。由于气候变化，世界变得越来越热，越来越干燥，人口增长迅速，据预测，到2050年，我们需要将粮食产量提高50% - 70%，才能养活预计的90 - 100亿人口。我们的研究旨在利用一种自然发生的超强光合作用适应，称为天冬酸代谢（CAM）。这种适应可以提高植物的水分利用效率，远远超过当今任何主要粮食作物物种，如水稻、小麦或玉米。通过对Kalanchoë属CAM模型物种的基因组和转录组进行解码，并开展功能基因组学研究，研究CAM候选基因的功能，建立CAM在C3作物中工程化的最小部件清单，以提高作物的水分利用效率和光合作用。该项目将利用我们最近的发现，通过使用转基因方法来关闭或打开与CAM相关的基因，和/或使用报告基因构建来探索基因调控。特别是，我们试图了解内源性生物钟（生物体用来优化其生物化学相对于每日光/暗周期的内部计时器）如何向CAM系统发出信号，以优化在黑暗和光明中分别发生的步骤。