CORE--ELECTRON MICROSCOPY

核心--电子显微镜

• 批准号: 7055788
• 负责人: George B Witman
• 金额: $ 5.22万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-11 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7055788
• 关键词: bioimaging /biomedical imaging; biomedical facility; diabetes mellitus; electron microscopy; scanning electron microscopy; tissue /cell preparation; transmission electron microscopy

The goal of the DERC Electron Microscopy (EM) Core Facility is to provide complete, first-rate EM service to DERC researchers at UMMS. The Facility is centrally located in a newly renovated suite in the basement of the Basic and Clinical Sciences Building. It is directed by Drs. George Witman and Roger Craig, and managed on a day-to-day basis by Dr. Gregory Hendricks, a full-time professional electron microscopist with extensive experience in virtually all EM methods. Equipment includes Philips CM120, CM12 and CM10 transmission electron microscopes and an ETEC Autoscan scanning electron microscope, a Balzers BAF400T freeze-etching system; three Reichert Ultracut E cryo-ultramicrotomes, and other equipment for specimen preparation. Techniques include immuno-EM, cryo-EM, and preparation of specimens by conventional thin sectioning, negative staining, metal shadowing, cryoultramicrotomy, rapid freezing, freeze substitution, freeze-fracture/freeze-etch, and critical point drying. Dr. Hendricks, aided by an assistant, can provide all services from specimen preparation to printing of electron micrographs. Alternatively, investigators may use Facility reagents and equipment to carry out their own specimen preparation and examination, with assistance from Facility staff as needed. Consequently, the Facility works just as well for users with no EM experience as for those with considerable EM experience. By centralizing all aspects of EM in this facility, the DERC provides its members with the best possible EM services in the most cost- and time-effective manner. The Facility, which is open to all UMMS researchers, is supported by a combination of institutional funds and user chargebacks; DERC investigators are charged at one-half the rate for non-affiliated UMMS researchers. Many DERC members use EM as an integral part of their research, and DERC members have published numerous papers that report results obtained in the EM Core Facility.

DERC电子显微镜（EM）核心设施的目标是为UMMS的DERC研究人员提供完整，一流的EM服务。该设施位于基础和临床科学大楼地下室新装修的套房内。它由乔治威特曼和罗杰克雷格博士指导，并由格雷戈里亨德里克斯博士管理，他是一位全职专业电子显微镜学家，在几乎所有的EM方法方面都有丰富的经验。设备包括Philips CM 120、CM 12和CM 10透射电子显微镜和一台ETEC Autoscan扫描电子显微镜、一台巴尔泽BAF 400 T冷冻蚀刻系统、三台Reichert Ultracut E冷冻超薄切片机和其他标本制备设备。技术包括免疫电镜、冷冻电镜和常规薄层扫描法制备标本。 切片、负染色、金属阴影、冷冻切片、快速冷冻、冷冻置换、冷冻断裂/冷冻蚀刻和临界点干燥。Hendricks博士在一名助手的帮助下，可以提供从样品制备到电子显微照片打印的所有服务。或者，研究者可以使用机构试剂和设备进行自己的样本制备和检查，必要时由机构工作人员提供协助。因此，该设施对于没有EM经验的用户和具有相当EM经验的用户同样有效。通过将EM的所有方面集中在该设施中，DERC 以最具成本效益和时间效益的方式为其会员提供最佳的EM服务。该设施向所有UMMS研究人员开放，由机构资金和用户退款相结合提供支持; DERC研究人员的费用是非附属UMMS研究人员的一半。许多DERC成员将EM作为其研究的一个组成部分，DERC成员发表了许多论文，报告了EM核心设施中获得的结果。