CORE--ELECTRON MICROSCOPY

DERC电子显微镜（EM）核心设施的目标是为UMMS的DERC研究人员提供完整的，一流的EM服务。该设施位于基础和临床科学大楼地下室新装修的套房的中心。它是由博士指导。George Witman和Roger Craig，并由Gregory Hendricks博士负责日常管理，Gregory Hendricks博士是一位全职的专业电子显微镜学家，在几乎所有的电子显微镜方法中都有丰富的经验。设备包括飞利浦CM120、CM12和CM10透射电子显微镜和ETEC Autoscan扫描电子显微镜，巴尔查斯BAF400T冷冻蚀刻系统；3台Reichert Ultracut E冷冻-超显微切片机，以及其他标本制备设备。技术包括免疫电镜、低温电镜和常规薄法制备标本

The goal of the DERC Electron Microscopy (EM) Core Facility is to provide complete, first-rate EM service to DERC researchers at UMMS. The Facility is centrally located in a newly renovated suite in the basement of the Basic and Clinical Sciences Building. It is directed by Drs. George Witman and Roger Craig, and managed on a day-to-day basis by Dr. Gregory Hendricks, a full-time professional electron microscopist with extensive experience in virtually all EM methods. Equipment includes Philips CM120, CM12 and CM10 transmission electron microscopes and an ETEC Autoscan scanning electron microscope, a Balzers BAF400T freeze-etching system; three Reichert Ultracut E cryo-ultramicrotomes, and other equipment for specimen preparation. Techniques include immuno-EM, cryo-EM, and preparation of specimens by conventional thin sectioning, negative staining, metal shadowing, cryoultramicrotomy, rapid freezing, freeze substitution, freeze-fracture/freeze-etch, and critical point drying. Dr. Hendricks, aided by an assistant, can provide all services from specimen preparation to printing of electron micrographs. Alternatively, investigators may use Facility reagents and equipment to carry out their own specimen preparation and examination, with assistance from Facility staff as needed. Consequently, the Facility works just as well for users with no EM experience as for those with considerable EM experience. By centralizing all aspects of EM in this facility, the DERC provides its members with the best possible EM services in the most cost- and time-effective manner. The Facility, which is open to all UMMS researchers, is supported by a combination of institutional funds and user chargebacks; DERC investigators are charged at one-half the rate for non-affiliated UMMS researchers. Many DERC members use EM as an integral part of their research, and DERC members have published numerous papers that report results obtained in the EM Core Facility.